Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells

Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells

The ability to edit the genome is essential for many state-of-the-art experimental paradigms. Since DNA breaks stimulate repair, they can be exploited to target site-specific integration. The clustered, regularly interspaced, short palindromic repeats (CRISPR)/cas9 system from Streptococcus pyogenes...

Full description

Bibliographic Details
Main Authors: Böttcher, Romy, Hollmann, Manuel, Merk, Karin, Nitschko, Volker, Obermaier, Christina, Philippou-Massier, Julia, Wieland, Isabella, Gaul, Ulrike, Förstemann, Klaus
Format: Online
Language:English
Published: Oxford University Press 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066747/

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066747/

Similar Items