Impact of Hfq on the Bacillus subtilis Transcriptome

The RNA chaperone Hfq acts as a central player in post-transcriptional gene regulation in several Gram-negative Bacteria, whereas comparatively little is known about its role in Gram-positive Bacteria. Here, we studied the function of Hfq in Bacillus subtilis, and show that it confers a survival adv...

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Main Authors:	Hämmerle, Hermann, Amman, Fabian, Večerek, Branislav, Stülke, Jörg, Hofacker, Ivo, Bläsi, Udo
Format:	Online
Language:	English
Published:	Public Library of Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059632/

id	pubmed-4059632
recordtype	oai_dc
spelling	pubmed-40596322014-06-19 Impact of Hfq on the Bacillus subtilis Transcriptome Hämmerle, Hermann Amman, Fabian Večerek, Branislav Stülke, Jörg Hofacker, Ivo Bläsi, Udo Research Article The RNA chaperone Hfq acts as a central player in post-transcriptional gene regulation in several Gram-negative Bacteria, whereas comparatively little is known about its role in Gram-positive Bacteria. Here, we studied the function of Hfq in Bacillus subtilis, and show that it confers a survival advantage. A comparative transcriptome analysis revealed mRNAs with a differential abundance that are governed by the ResD-ResE system required for aerobic and anaerobic respiration. Expression of resD was found to be up-regulated in the hfq− strain. Furthermore, several genes of the GerE and ComK regulons were de-regulated in the hfq− background. Surprisingly, only six out of >100 known and predicted small RNAs (sRNAs) showed altered abundance in the absence of Hfq. Moreover, Hfq positively affected the transcript abundance of genes encoding type I toxin-antitoxin systems. Taken the moderate effect on sRNA levels and mRNAs together, it seems rather unlikely that Hfq plays a central role in RNA transactions in Bacillus subtilis. Public Library of Science 2014-06-16 /pmc/articles/PMC4059632/ /pubmed/24932523 http://dx.doi.org/10.1371/journal.pone.0098661 Text en © 2014 Hämmerle et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Hämmerle, Hermann Amman, Fabian Večerek, Branislav Stülke, Jörg Hofacker, Ivo Bläsi, Udo
spellingShingle	Hämmerle, Hermann Amman, Fabian Večerek, Branislav Stülke, Jörg Hofacker, Ivo Bläsi, Udo Impact of Hfq on the Bacillus subtilis Transcriptome
author_facet	Hämmerle, Hermann Amman, Fabian Večerek, Branislav Stülke, Jörg Hofacker, Ivo Bläsi, Udo
author_sort	Hämmerle, Hermann
title	Impact of Hfq on the Bacillus subtilis Transcriptome
title_short	Impact of Hfq on the Bacillus subtilis Transcriptome
title_full	Impact of Hfq on the Bacillus subtilis Transcriptome
title_fullStr	Impact of Hfq on the Bacillus subtilis Transcriptome
title_full_unstemmed	Impact of Hfq on the Bacillus subtilis Transcriptome
title_sort	impact of hfq on the bacillus subtilis transcriptome
description	The RNA chaperone Hfq acts as a central player in post-transcriptional gene regulation in several Gram-negative Bacteria, whereas comparatively little is known about its role in Gram-positive Bacteria. Here, we studied the function of Hfq in Bacillus subtilis, and show that it confers a survival advantage. A comparative transcriptome analysis revealed mRNAs with a differential abundance that are governed by the ResD-ResE system required for aerobic and anaerobic respiration. Expression of resD was found to be up-regulated in the hfq− strain. Furthermore, several genes of the GerE and ComK regulons were de-regulated in the hfq− background. Surprisingly, only six out of >100 known and predicted small RNAs (sRNAs) showed altered abundance in the absence of Hfq. Moreover, Hfq positively affected the transcript abundance of genes encoding type I toxin-antitoxin systems. Taken the moderate effect on sRNA levels and mRNAs together, it seems rather unlikely that Hfq plays a central role in RNA transactions in Bacillus subtilis.
publisher	Public Library of Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059632/
_version_	1612102064177938432

Impact of Hfq on the Bacillus subtilis Transcriptome

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