Campylobacter jejuni in commercial eggs

This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commerc...

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Main Authors:	Fonseca, Belchiolina Beatriz, Beletti, Marcelo Emílio, de Melo, Roberta Torres, Mendonça, Eliane Pereira, Coelho, Letícia Ríspoli, Nalevaiko, Priscila Christen, Rossi, Daise Aparecida
Format:	Online
Language:	English
Published:	Sociedade Brasileira de Microbiologia 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059329/

id	pubmed-4059329
recordtype	oai_dc
spelling	pubmed-40593292014-06-19 Campylobacter jejuni in commercial eggs Fonseca, Belchiolina Beatriz Beletti, Marcelo Emílio de Melo, Roberta Torres Mendonça, Eliane Pereira Coelho, Letícia Ríspoli Nalevaiko, Priscila Christen Rossi, Daise Aparecida Research Paper This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health. Sociedade Brasileira de Microbiologia 2014-05-19 /pmc/articles/PMC4059329/ /pubmed/24948916 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Fonseca, Belchiolina Beatriz Beletti, Marcelo Emílio de Melo, Roberta Torres Mendonça, Eliane Pereira Coelho, Letícia Ríspoli Nalevaiko, Priscila Christen Rossi, Daise Aparecida
spellingShingle	Fonseca, Belchiolina Beatriz Beletti, Marcelo Emílio de Melo, Roberta Torres Mendonça, Eliane Pereira Coelho, Letícia Ríspoli Nalevaiko, Priscila Christen Rossi, Daise Aparecida Campylobacter jejuni in commercial eggs
author_facet	Fonseca, Belchiolina Beatriz Beletti, Marcelo Emílio de Melo, Roberta Torres Mendonça, Eliane Pereira Coelho, Letícia Ríspoli Nalevaiko, Priscila Christen Rossi, Daise Aparecida
author_sort	Fonseca, Belchiolina Beatriz
title	Campylobacter jejuni in commercial eggs
title_short	Campylobacter jejuni in commercial eggs
title_full	Campylobacter jejuni in commercial eggs
title_fullStr	Campylobacter jejuni in commercial eggs
title_full_unstemmed	Campylobacter jejuni in commercial eggs
title_sort	campylobacter jejuni in commercial eggs
description	This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health.
publisher	Sociedade Brasileira de Microbiologia
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059329/
_version_	1612102000211656704

Campylobacter jejuni in commercial eggs

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