Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats

The present study was designed to probe the effects of Huperzine A (HupA) on diabetes-associated cognitive decline (DACD) using a streptozotocin (STZ)-injected rat model. Diabetic rats were treated with HupA (0.05 and 0.1 mg/kg) for seven weeks. Memory functions were evaluated by the water maze test...

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Main Authors:	Mao, Xiao-Yuan, Cao, Dan-Feng, Li, Xi, Yin, Ji-Ye, Wang, Zhi-Bin, Zhang, Ying, Mao, Chen-Xue, Zhou, Hong-Hao, Liu, Zhao-Qian
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057698/

id	pubmed-4057698
recordtype	oai_dc
spelling	pubmed-40576982014-06-16 Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats Mao, Xiao-Yuan Cao, Dan-Feng Li, Xi Yin, Ji-Ye Wang, Zhi-Bin Zhang, Ying Mao, Chen-Xue Zhou, Hong-Hao Liu, Zhao-Qian Article The present study was designed to probe the effects of Huperzine A (HupA) on diabetes-associated cognitive decline (DACD) using a streptozotocin (STZ)-injected rat model. Diabetic rats were treated with HupA (0.05 and 0.1 mg/kg) for seven weeks. Memory functions were evaluated by the water maze test. Nissl staining was selected for detecting neuronal loss. Protein and mRNA levels of brain-derived neurotrophic factor (BDNF) were analyzed by ELISA and real-time PCR, respectively. The activities of choline acetylase (ChAT), Acetylcholinesterase (AChE), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), NF-κB p65 unit, TNF-α, IL-1β, IL-6 and caspase-3 were measured using corresponding kits. After seven weeks, diabetic rats exhibited remarkable reductions in: body weight, percentage of time spent in target quadrant, number of times crossing the platform, ChAT and BDNF levels, SOD, GSH-Px and CAT accompanied with increases in neuronal damage, plasma glucose levels, escape latency, mean path length, AChE, MDA level as well as CAT, NF-κB p65 unit, TNF-α, IL-1β, IL-6 and caspase-3 in cerebral cortex and hippocampus. Supplementation with HupA significantly and dose-dependently reversed the corresponding values in diabetes. It is concluded that HupA ameliorates DACD via modulating BDNF, oxidative stress, inflammation and apoptosis. Molecular Diversity Preservation International (MDPI) 2014-05-05 /pmc/articles/PMC4057698/ /pubmed/24857910 http://dx.doi.org/10.3390/ijms15057667 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Mao, Xiao-Yuan Cao, Dan-Feng Li, Xi Yin, Ji-Ye Wang, Zhi-Bin Zhang, Ying Mao, Chen-Xue Zhou, Hong-Hao Liu, Zhao-Qian
spellingShingle	Mao, Xiao-Yuan Cao, Dan-Feng Li, Xi Yin, Ji-Ye Wang, Zhi-Bin Zhang, Ying Mao, Chen-Xue Zhou, Hong-Hao Liu, Zhao-Qian Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats
author_facet	Mao, Xiao-Yuan Cao, Dan-Feng Li, Xi Yin, Ji-Ye Wang, Zhi-Bin Zhang, Ying Mao, Chen-Xue Zhou, Hong-Hao Liu, Zhao-Qian
author_sort	Mao, Xiao-Yuan
title	Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats
title_short	Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats
title_full	Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats
title_fullStr	Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats
title_full_unstemmed	Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats
title_sort	huperzine a ameliorates cognitive deficits in streptozotocin-induced diabetic rats
description	The present study was designed to probe the effects of Huperzine A (HupA) on diabetes-associated cognitive decline (DACD) using a streptozotocin (STZ)-injected rat model. Diabetic rats were treated with HupA (0.05 and 0.1 mg/kg) for seven weeks. Memory functions were evaluated by the water maze test. Nissl staining was selected for detecting neuronal loss. Protein and mRNA levels of brain-derived neurotrophic factor (BDNF) were analyzed by ELISA and real-time PCR, respectively. The activities of choline acetylase (ChAT), Acetylcholinesterase (AChE), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), NF-κB p65 unit, TNF-α, IL-1β, IL-6 and caspase-3 were measured using corresponding kits. After seven weeks, diabetic rats exhibited remarkable reductions in: body weight, percentage of time spent in target quadrant, number of times crossing the platform, ChAT and BDNF levels, SOD, GSH-Px and CAT accompanied with increases in neuronal damage, plasma glucose levels, escape latency, mean path length, AChE, MDA level as well as CAT, NF-κB p65 unit, TNF-α, IL-1β, IL-6 and caspase-3 in cerebral cortex and hippocampus. Supplementation with HupA significantly and dose-dependently reversed the corresponding values in diabetes. It is concluded that HupA ameliorates DACD via modulating BDNF, oxidative stress, inflammation and apoptosis.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057698/
_version_	1612101390084079616

Huperzine A Ameliorates Cognitive Deficits in Streptozotocin-Induced Diabetic Rats

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