Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells

Vasohibin-1 (VASH1) is a VEGF-inducible gene of endothelial cells (ECs) that acts as a negative feedback regulator of angiogenesis. To further characterize the function of VASH1, we transfected human VASH1 gene into the mouse EC line MS1, established stable VASH1 expressing clones, and determined ge...

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Main Authors:	Miyashita, Hiroki, Suzuki, Hirotada, Ohkuchi, Akihide, Sato, Yasufumi
Format:	Online
Language:	English
Published:	MDPI 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055955/

id	pubmed-4055955
recordtype	oai_dc
spelling	pubmed-40559552014-06-13 Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells Miyashita, Hiroki Suzuki, Hirotada Ohkuchi, Akihide Sato, Yasufumi Article Vasohibin-1 (VASH1) is a VEGF-inducible gene of endothelial cells (ECs) that acts as a negative feedback regulator of angiogenesis. To further characterize the function of VASH1, we transfected human VASH1 gene into the mouse EC line MS1, established stable VASH1 expressing clones, and determined gene alteration by cDNA microarray analysis. Among the various angiogenesis-related genes, vascular endothelial growth factor type 1 receptor (VEGFR-1) and its alternative spliced form, soluble VEGFR1 (sVEGFR-1), were found to be the most significantly down-regulated genes. Transient overexpression of VASH1 in human umbilical vein endothelial cells confirmed the down-regulation of VEGFR-1 and sVEGFR-1. sVEGFR-1 is a decoy receptor for VEGF and inhibits angiogenesis. Interestingly, when sVEGFR-1 was overexpressed in ECs, it inhibited the expression of VASH1 in turn. These results suggest that VASH1 and sVEGFR-1, two angiogenesis inhibitors, mutually balance their expressions in ECs. MDPI 2011-05-31 /pmc/articles/PMC4055955/ http://dx.doi.org/10.3390/ph4060782 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Miyashita, Hiroki Suzuki, Hirotada Ohkuchi, Akihide Sato, Yasufumi
spellingShingle	Miyashita, Hiroki Suzuki, Hirotada Ohkuchi, Akihide Sato, Yasufumi Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells
author_facet	Miyashita, Hiroki Suzuki, Hirotada Ohkuchi, Akihide Sato, Yasufumi
author_sort	Miyashita, Hiroki
title	Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells
title_short	Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells
title_full	Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells
title_fullStr	Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells
title_full_unstemmed	Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells
title_sort	mutual balance between vasohibin-1 and soluble vegfr-1 in endothelial cells
description	Vasohibin-1 (VASH1) is a VEGF-inducible gene of endothelial cells (ECs) that acts as a negative feedback regulator of angiogenesis. To further characterize the function of VASH1, we transfected human VASH1 gene into the mouse EC line MS1, established stable VASH1 expressing clones, and determined gene alteration by cDNA microarray analysis. Among the various angiogenesis-related genes, vascular endothelial growth factor type 1 receptor (VEGFR-1) and its alternative spliced form, soluble VEGFR1 (sVEGFR-1), were found to be the most significantly down-regulated genes. Transient overexpression of VASH1 in human umbilical vein endothelial cells confirmed the down-regulation of VEGFR-1 and sVEGFR-1. sVEGFR-1 is a decoy receptor for VEGF and inhibits angiogenesis. Interestingly, when sVEGFR-1 was overexpressed in ECs, it inhibited the expression of VASH1 in turn. These results suggest that VASH1 and sVEGFR-1, two angiogenesis inhibitors, mutually balance their expressions in ECs.
publisher	MDPI
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055955/
_version_	1612100667652964352

Mutual Balance between Vasohibin-1 and Soluble VEGFR-1 in Endothelial Cells

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