Cardioprotective Effect of Betulinic Acid on Myocardial Ischemia Reperfusion Injury in Rats

Objectives. This study aims to investigate the effect of betulinic acid (BA) on myocardial ischemia reperfusion/injury in an open-chest anesthetized rat model. Methods. The model was induced by 30 minutes left anterior descending occlusion followed by 2 hours reperfusion. There are six groups in ou...

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Main Authors:	Xia, Anzhou, Xue, Zhi, Li, Yong, Wang, Wei, Xia, Jieyun, Wei, Tiantian, Cao, Jing, Zhou, Weidong
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055472/

id	pubmed-4055472
recordtype	oai_dc
spelling	pubmed-40554722014-06-24 Cardioprotective Effect of Betulinic Acid on Myocardial Ischemia Reperfusion Injury in Rats Xia, Anzhou Xue, Zhi Li, Yong Wang, Wei Xia, Jieyun Wei, Tiantian Cao, Jing Zhou, Weidong Research Article Objectives. This study aims to investigate the effect of betulinic acid (BA) on myocardial ischemia reperfusion/injury in an open-chest anesthetized rat model. Methods. The model was induced by 30 minutes left anterior descending occlusion followed by 2 hours reperfusion. There are six groups in our present study: sham operation group, ischemia/reperfusion group, low-dosage BA group, medium-dosage BA group, high-dosage BA group, and fosinopril sodium group. Rats in the latter four groups were administrated with BA (50, 100, and 200 mg/kg, i.g.) or fosinopril sodium (10 mg/kg, i.g.) once a day for 7 days before operation, respectively. Rats in the former two groups were given the same volume of vehicle (0.5% CMC-Na, i.g.). During the operation, cardiac function was continuously monitored. Serum LDH and CK were measured with colorimetric assays. The expression of Bcl-2 and Bax and the apoptosis of cardiomyocytes were investigated with western blot and TUNEL assay, respectively. Results. Pretreatment with BA improved cardiac function and attenuated LDH and CK activities compared with IR group. Further investigation demonstrated that the expression of Bcl-2 and Bax and TUNEL assay was in line with the above results. Conclusion. BA may reduce the release of LDH and CK, prevent cardiomyocytes apoptosis, and eventually alleviate the extent of the myocardial ischemia/reperfusion injury. Hindawi Publishing Corporation 2014 2014-05-21 /pmc/articles/PMC4055472/ /pubmed/24963326 http://dx.doi.org/10.1155/2014/573745 Text en Copyright © 2014 Anzhou Xia et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Xia, Anzhou Xue, Zhi Li, Yong Wang, Wei Xia, Jieyun Wei, Tiantian Cao, Jing Zhou, Weidong
spellingShingle	Xia, Anzhou Xue, Zhi Li, Yong Wang, Wei Xia, Jieyun Wei, Tiantian Cao, Jing Zhou, Weidong Cardioprotective Effect of Betulinic Acid on Myocardial Ischemia Reperfusion Injury in Rats
author_facet	Xia, Anzhou Xue, Zhi Li, Yong Wang, Wei Xia, Jieyun Wei, Tiantian Cao, Jing Zhou, Weidong
author_sort	Xia, Anzhou
title	Cardioprotective Effect of Betulinic Acid on Myocardial Ischemia Reperfusion Injury in Rats
title_short	Cardioprotective Effect of Betulinic Acid on Myocardial Ischemia Reperfusion Injury in Rats
title_full	Cardioprotective Effect of Betulinic Acid on Myocardial Ischemia Reperfusion Injury in Rats
title_fullStr	Cardioprotective Effect of Betulinic Acid on Myocardial Ischemia Reperfusion Injury in Rats
title_full_unstemmed	Cardioprotective Effect of Betulinic Acid on Myocardial Ischemia Reperfusion Injury in Rats
title_sort	cardioprotective effect of betulinic acid on myocardial ischemia reperfusion injury in rats
description	Objectives. This study aims to investigate the effect of betulinic acid (BA) on myocardial ischemia reperfusion/injury in an open-chest anesthetized rat model. Methods. The model was induced by 30 minutes left anterior descending occlusion followed by 2 hours reperfusion. There are six groups in our present study: sham operation group, ischemia/reperfusion group, low-dosage BA group, medium-dosage BA group, high-dosage BA group, and fosinopril sodium group. Rats in the latter four groups were administrated with BA (50, 100, and 200 mg/kg, i.g.) or fosinopril sodium (10 mg/kg, i.g.) once a day for 7 days before operation, respectively. Rats in the former two groups were given the same volume of vehicle (0.5% CMC-Na, i.g.). During the operation, cardiac function was continuously monitored. Serum LDH and CK were measured with colorimetric assays. The expression of Bcl-2 and Bax and the apoptosis of cardiomyocytes were investigated with western blot and TUNEL assay, respectively. Results. Pretreatment with BA improved cardiac function and attenuated LDH and CK activities compared with IR group. Further investigation demonstrated that the expression of Bcl-2 and Bax and TUNEL assay was in line with the above results. Conclusion. BA may reduce the release of LDH and CK, prevent cardiomyocytes apoptosis, and eventually alleviate the extent of the myocardial ischemia/reperfusion injury.
publisher	Hindawi Publishing Corporation
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055472/
_version_	1612100429386088448

Cardioprotective Effect of Betulinic Acid on Myocardial Ischemia Reperfusion Injury in Rats

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