The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination

Fermentative hydrogen production from wastes has many advantages compared to various chemical methods. Methodology for characterizing the hydrogen-producing activity of anaerobic mixed cultures is essential for monitoring reactor operation in fermentative hydrogen production, however there is lack o...

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Main Authors:	Mu, Yang, Yang, Hou-Yun, Wang, Ya-Zhou, He, Chuan-Shu, Zhao, Quan-Bao, Wang, Yi, Yu, Han-Qing
Format:	Online
Language:	English
Published:	Nature Publishing Group 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050381/

id	pubmed-4050381
recordtype	oai_dc
spelling	pubmed-40503812014-06-12 The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination Mu, Yang Yang, Hou-Yun Wang, Ya-Zhou He, Chuan-Shu Zhao, Quan-Bao Wang, Yi Yu, Han-Qing Article Fermentative hydrogen production from wastes has many advantages compared to various chemical methods. Methodology for characterizing the hydrogen-producing activity of anaerobic mixed cultures is essential for monitoring reactor operation in fermentative hydrogen production, however there is lack of such kind of standardized methodologies. In the present study, a new index, i.e., the maximum specific hydrogen-producing activity (SHAm) of anaerobic mixed cultures, was proposed, and consequently a reliable and simple method, named SHAm test, was developed to determine it. Furthermore, the influences of various parameters on the SHAm value determination of anaerobic mixed cultures were evaluated. Additionally, this SHAm assay was tested for different types of substrates and bacterial inocula. Our results demonstrate that this novel SHAm assay was a rapid, accurate and simple methodology for determining the hydrogen-producing activity of anaerobic mixed cultures. Thus, application of this approach is beneficial to establishing a stable anaerobic hydrogen-producing system. Nature Publishing Group 2014-06-10 /pmc/articles/PMC4050381/ /pubmed/24912488 http://dx.doi.org/10.1038/srep05239 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Mu, Yang Yang, Hou-Yun Wang, Ya-Zhou He, Chuan-Shu Zhao, Quan-Bao Wang, Yi Yu, Han-Qing
spellingShingle	Mu, Yang Yang, Hou-Yun Wang, Ya-Zhou He, Chuan-Shu Zhao, Quan-Bao Wang, Yi Yu, Han-Qing The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination
author_facet	Mu, Yang Yang, Hou-Yun Wang, Ya-Zhou He, Chuan-Shu Zhao, Quan-Bao Wang, Yi Yu, Han-Qing
author_sort	Mu, Yang
title	The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination
title_short	The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination
title_full	The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination
title_fullStr	The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination
title_full_unstemmed	The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination
title_sort	maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination
description	Fermentative hydrogen production from wastes has many advantages compared to various chemical methods. Methodology for characterizing the hydrogen-producing activity of anaerobic mixed cultures is essential for monitoring reactor operation in fermentative hydrogen production, however there is lack of such kind of standardized methodologies. In the present study, a new index, i.e., the maximum specific hydrogen-producing activity (SHAm) of anaerobic mixed cultures, was proposed, and consequently a reliable and simple method, named SHAm test, was developed to determine it. Furthermore, the influences of various parameters on the SHAm value determination of anaerobic mixed cultures were evaluated. Additionally, this SHAm assay was tested for different types of substrates and bacterial inocula. Our results demonstrate that this novel SHAm assay was a rapid, accurate and simple methodology for determining the hydrogen-producing activity of anaerobic mixed cultures. Thus, application of this approach is beneficial to establishing a stable anaerobic hydrogen-producing system.
publisher	Nature Publishing Group
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050381/
_version_	1612098560602406912

The maximum specific hydrogen-producing activity of anaerobic mixed cultures: definition and determination

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