Specialized Transduction Designed for Precise High-Throughput Unmarked Deletions in Mycobacterium tuberculosis

Specialized Transduction Designed for Precise High-Throughput Unmarked Deletions in Mycobacterium tuberculosis

Specialized transduction has proven to be useful for generating deletion mutants in most mycobacteria, including virulent Mycobacterium tuberculosis. We have improved this system by developing (i) a single-step strategy for the construction of allelic exchange substrates (AES), (ii) a temperature-se...

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Bibliographic Details
Main Authors: Jain, Paras, Hsu, Tsungda, Arai, Masayoshi, Biermann, Karolin, Thaler, David S., Nguyen, Andrew, González, Pablo A., Tufariello, Joann M., Kriakov, Jordan, Chen, Bing, Larsen, Michelle H., Jacobs, William R.
Format: Online
Language:English
Published: American Society of Microbiology 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049104/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049104/

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