The role of N-glycosylation in kiwi allergy

The physical, biochemical, and immunological characteristics of plant allergens have been widely studied, but no definite conclusion has been reached about what actually makes a protein an allergen. In this sense, N-glycosylation is an exclusive characteristic of plant allergens not present in mamma...

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Main Authors:	Garrido-Arandia, María, Murua-García, Amaya, Palacin, Aranzazu, Tordesillas, Leticia, Gómez-Casado, Cristina, Blanca-Lopez, Natalia, Ramos, Tania, Canto, Gabriela, Blanco, Carlos, Cuesta-Herranz, Javier, Sánchez-Monge, Rosa, Pacios, Luis F, Díaz Perales, Araceli
Format:	Online
Language:	English
Published:	BlackWell Publishing Ltd 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4048612/

id	pubmed-4048612
recordtype	oai_dc
spelling	pubmed-40486122014-06-16 The role of N-glycosylation in kiwi allergy Garrido-Arandia, María Murua-García, Amaya Palacin, Aranzazu Tordesillas, Leticia Gómez-Casado, Cristina Blanca-Lopez, Natalia Ramos, Tania Canto, Gabriela Blanco, Carlos Cuesta-Herranz, Javier Sánchez-Monge, Rosa Pacios, Luis F Díaz Perales, Araceli Original Research The physical, biochemical, and immunological characteristics of plant allergens have been widely studied, but no definite conclusion has been reached about what actually makes a protein an allergen. In this sense, N-glycosylation is an exclusive characteristic of plant allergens not present in mammals and it could be implied in allergenic sensitization. With this aim, we evaluated and compared the allergenic activity of the protein fraction and the N-glycan fraction of the thaumatin-like protein and the main kiwi allergen, Act d 2. The natural allergen, Act d 2, was deglycosylated by trifluoromethanesulfonic acid treatment; the N-glycan fraction was obtained by extended treatment with proteinase K. N-glycan- and protein- fractions were recognized by specific IgE of kiwi-allergic patients. By contrast, the sugar moiety showed a reduced capacity to activate basophils and T cells, but not dendritic cells derived from patients' monocytes. Related to this, the production of cytokines such as IL6 and IL10 was increased by the incubation of dendritic cells with sugar moiety. Thus, the sugar moiety plays a significant role in sensitization, inducing the activation of antigen-presenting cells, but it is the protein fraction that is responsible for the allergic reactions. BlackWell Publishing Ltd 2014-05 2014-03-19 /pmc/articles/PMC4048612/ /pubmed/24936296 http://dx.doi.org/10.1002/fsn3.99 Text en © 2014 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Garrido-Arandia, María Murua-García, Amaya Palacin, Aranzazu Tordesillas, Leticia Gómez-Casado, Cristina Blanca-Lopez, Natalia Ramos, Tania Canto, Gabriela Blanco, Carlos Cuesta-Herranz, Javier Sánchez-Monge, Rosa Pacios, Luis F Díaz Perales, Araceli
spellingShingle	Garrido-Arandia, María Murua-García, Amaya Palacin, Aranzazu Tordesillas, Leticia Gómez-Casado, Cristina Blanca-Lopez, Natalia Ramos, Tania Canto, Gabriela Blanco, Carlos Cuesta-Herranz, Javier Sánchez-Monge, Rosa Pacios, Luis F Díaz Perales, Araceli The role of N-glycosylation in kiwi allergy
author_facet	Garrido-Arandia, María Murua-García, Amaya Palacin, Aranzazu Tordesillas, Leticia Gómez-Casado, Cristina Blanca-Lopez, Natalia Ramos, Tania Canto, Gabriela Blanco, Carlos Cuesta-Herranz, Javier Sánchez-Monge, Rosa Pacios, Luis F Díaz Perales, Araceli
author_sort	Garrido-Arandia, María
title	The role of N-glycosylation in kiwi allergy
title_short	The role of N-glycosylation in kiwi allergy
title_full	The role of N-glycosylation in kiwi allergy
title_fullStr	The role of N-glycosylation in kiwi allergy
title_full_unstemmed	The role of N-glycosylation in kiwi allergy
title_sort	role of n-glycosylation in kiwi allergy
description	The physical, biochemical, and immunological characteristics of plant allergens have been widely studied, but no definite conclusion has been reached about what actually makes a protein an allergen. In this sense, N-glycosylation is an exclusive characteristic of plant allergens not present in mammals and it could be implied in allergenic sensitization. With this aim, we evaluated and compared the allergenic activity of the protein fraction and the N-glycan fraction of the thaumatin-like protein and the main kiwi allergen, Act d 2. The natural allergen, Act d 2, was deglycosylated by trifluoromethanesulfonic acid treatment; the N-glycan fraction was obtained by extended treatment with proteinase K. N-glycan- and protein- fractions were recognized by specific IgE of kiwi-allergic patients. By contrast, the sugar moiety showed a reduced capacity to activate basophils and T cells, but not dendritic cells derived from patients' monocytes. Related to this, the production of cytokines such as IL6 and IL10 was increased by the incubation of dendritic cells with sugar moiety. Thus, the sugar moiety plays a significant role in sensitization, inducing the activation of antigen-presenting cells, but it is the protein fraction that is responsible for the allergic reactions.
publisher	BlackWell Publishing Ltd
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4048612/
_version_	1612098059570774016

The role of N-glycosylation in kiwi allergy

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