Effects of dexmedetomidine on the deformability of erythrocytes in vitro and in anesthesia

The current study aimed to evaluate the impact of clinically relevant concentrations of dexmedetomidine on the deformability of erythrocytes in vitro and the effects of dexmedetomidine on the deformability of erythrocytes in patients undergoing laparoscopic cholecystectomy. Erythrocyte suspensions o...

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Main Authors:	YANG, XIAO-MING, LIU, JUN, JI, JUN, XIE, JIE
Format:	Online
Language:	English
Published:	D.A. Spandidos 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043598/

id	pubmed-4043598
recordtype	oai_dc
spelling	pubmed-40435982014-06-12 Effects of dexmedetomidine on the deformability of erythrocytes in vitro and in anesthesia YANG, XIAO-MING LIU, JUN JI, JUN XIE, JIE Articles The current study aimed to evaluate the impact of clinically relevant concentrations of dexmedetomidine on the deformability of erythrocytes in vitro and the effects of dexmedetomidine on the deformability of erythrocytes in patients undergoing laparoscopic cholecystectomy. Erythrocyte suspensions of different concentrations were divided into six groups: Control (group C); low, medium and high concentrations of dexmedetomidine (groups DL, DM and DH, respectively); yohimbine alone (group Y) and yohimbine mixed with dexmedetomidine (group YD). The suspensions were incubated in a thermostatic shaking incubator (50 rpm, 37°C) for 60 min. The nitric oxide (NO) concentrations and endothelial nitric oxide synthase (eNOS) activities of red blood cells and the erythrocyte deformability index (EI) were then measured. Patients (n=40) scheduled for laparoscopic cholecystectomy were randomly divided into a dexmedetomidine group (group A) and a control group (group B). The induction and maintenance of anesthesia in the two groups was identical. The EI and hematocrit (Hct) were assayed prior to anesthesia (T0) and following the surgery (T1). In the in vitro assay, the EI, the activity of eNOS and the NO concentration of the erythrocytes were significantly higher in groups DL, DM, DH and YD than in group C (P<0.05). In addition, the EI, the eNOS activity and NO concentration of the erythrocytes were higher in group DM than in group YD (P<0.05). In the patients, the EI value at T1 (0.90±0.04) was higher than at T0 (0.81±0.06) in group B (P<0.05). No statistically significant difference between the EI values at T0 and T1 was identified in group A (P>0.05). Dexmedetomidine treatment is able to improve the deformability of erythrocytes in vitro and in anesthesia. The improvement of erythrocyte deformability by dexmedetomidine may be partially associated with adrenergic receptors through activation of eNOS to enhance the concentration of NO in red blood cells. D.A. Spandidos 2014-06 2014-03-27 /pmc/articles/PMC4043598/ /pubmed/24926356 http://dx.doi.org/10.3892/etm.2014.1633 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	YANG, XIAO-MING LIU, JUN JI, JUN XIE, JIE
spellingShingle	YANG, XIAO-MING LIU, JUN JI, JUN XIE, JIE Effects of dexmedetomidine on the deformability of erythrocytes in vitro and in anesthesia
author_facet	YANG, XIAO-MING LIU, JUN JI, JUN XIE, JIE
author_sort	YANG, XIAO-MING
title	Effects of dexmedetomidine on the deformability of erythrocytes in vitro and in anesthesia
title_short	Effects of dexmedetomidine on the deformability of erythrocytes in vitro and in anesthesia
title_full	Effects of dexmedetomidine on the deformability of erythrocytes in vitro and in anesthesia
title_fullStr	Effects of dexmedetomidine on the deformability of erythrocytes in vitro and in anesthesia
title_full_unstemmed	Effects of dexmedetomidine on the deformability of erythrocytes in vitro and in anesthesia
title_sort	effects of dexmedetomidine on the deformability of erythrocytes in vitro and in anesthesia
description	The current study aimed to evaluate the impact of clinically relevant concentrations of dexmedetomidine on the deformability of erythrocytes in vitro and the effects of dexmedetomidine on the deformability of erythrocytes in patients undergoing laparoscopic cholecystectomy. Erythrocyte suspensions of different concentrations were divided into six groups: Control (group C); low, medium and high concentrations of dexmedetomidine (groups DL, DM and DH, respectively); yohimbine alone (group Y) and yohimbine mixed with dexmedetomidine (group YD). The suspensions were incubated in a thermostatic shaking incubator (50 rpm, 37°C) for 60 min. The nitric oxide (NO) concentrations and endothelial nitric oxide synthase (eNOS) activities of red blood cells and the erythrocyte deformability index (EI) were then measured. Patients (n=40) scheduled for laparoscopic cholecystectomy were randomly divided into a dexmedetomidine group (group A) and a control group (group B). The induction and maintenance of anesthesia in the two groups was identical. The EI and hematocrit (Hct) were assayed prior to anesthesia (T0) and following the surgery (T1). In the in vitro assay, the EI, the activity of eNOS and the NO concentration of the erythrocytes were significantly higher in groups DL, DM, DH and YD than in group C (P<0.05). In addition, the EI, the eNOS activity and NO concentration of the erythrocytes were higher in group DM than in group YD (P<0.05). In the patients, the EI value at T1 (0.90±0.04) was higher than at T0 (0.81±0.06) in group B (P<0.05). No statistically significant difference between the EI values at T0 and T1 was identified in group A (P>0.05). Dexmedetomidine treatment is able to improve the deformability of erythrocytes in vitro and in anesthesia. The improvement of erythrocyte deformability by dexmedetomidine may be partially associated with adrenergic receptors through activation of eNOS to enhance the concentration of NO in red blood cells.
publisher	D.A. Spandidos
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043598/
_version_	1612096015149563904

Effects of dexmedetomidine on the deformability of erythrocytes in vitro and in anesthesia

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