Changes in Abundance of Oral Microbiota Associated with Oral Cancer

Individual bacteria and shifts in the composition of the microbiome have been associated with human diseases including cancer. To investigate changes in the microbiome associated with oral cancers, we profiled cancers and anatomically matched contralateral normal tissue from the same patient by sequ...

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Main Authors:	Schmidt, Brian L., Kuczynski, Justin, Bhattacharya, Aditi, Huey, Bing, Corby, Patricia M., Queiroz, Erica L. S., Nightingale, Kira, Kerr, A. Ross, DeLacure, Mark D., Veeramachaneni, Ratna, Olshen, Adam B., Albertson, Donna G.
Format:	Online
Language:	English
Published:	Public Library of Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041887/

id	pubmed-4041887
recordtype	oai_dc
spelling	pubmed-40418872014-06-09 Changes in Abundance of Oral Microbiota Associated with Oral Cancer Schmidt, Brian L. Kuczynski, Justin Bhattacharya, Aditi Huey, Bing Corby, Patricia M. Queiroz, Erica L. S. Nightingale, Kira Kerr, A. Ross DeLacure, Mark D. Veeramachaneni, Ratna Olshen, Adam B. Albertson, Donna G. Research Article Individual bacteria and shifts in the composition of the microbiome have been associated with human diseases including cancer. To investigate changes in the microbiome associated with oral cancers, we profiled cancers and anatomically matched contralateral normal tissue from the same patient by sequencing 16S rDNA hypervariable region amplicons. In cancer samples from both a discovery and a subsequent confirmation cohort, abundance of Firmicutes (especially Streptococcus) and Actinobacteria (especially Rothia) was significantly decreased relative to contralateral normal samples from the same patient. Significant decreases in abundance of these phyla were observed for pre-cancers, but not when comparing samples from contralateral sites (tongue and floor of mouth) from healthy individuals. Weighted UniFrac principal coordinates analysis based on 12 taxa separated most cancers from other samples with greatest separation of node positive cases. These studies begin to develop a framework for exploiting the oral microbiome for monitoring oral cancer development, progression and recurrence. Public Library of Science 2014-06-02 /pmc/articles/PMC4041887/ /pubmed/24887397 http://dx.doi.org/10.1371/journal.pone.0098741 Text en © 2014 Schmidt et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Schmidt, Brian L. Kuczynski, Justin Bhattacharya, Aditi Huey, Bing Corby, Patricia M. Queiroz, Erica L. S. Nightingale, Kira Kerr, A. Ross DeLacure, Mark D. Veeramachaneni, Ratna Olshen, Adam B. Albertson, Donna G.
spellingShingle	Schmidt, Brian L. Kuczynski, Justin Bhattacharya, Aditi Huey, Bing Corby, Patricia M. Queiroz, Erica L. S. Nightingale, Kira Kerr, A. Ross DeLacure, Mark D. Veeramachaneni, Ratna Olshen, Adam B. Albertson, Donna G. Changes in Abundance of Oral Microbiota Associated with Oral Cancer
author_facet	Schmidt, Brian L. Kuczynski, Justin Bhattacharya, Aditi Huey, Bing Corby, Patricia M. Queiroz, Erica L. S. Nightingale, Kira Kerr, A. Ross DeLacure, Mark D. Veeramachaneni, Ratna Olshen, Adam B. Albertson, Donna G.
author_sort	Schmidt, Brian L.
title	Changes in Abundance of Oral Microbiota Associated with Oral Cancer
title_short	Changes in Abundance of Oral Microbiota Associated with Oral Cancer
title_full	Changes in Abundance of Oral Microbiota Associated with Oral Cancer
title_fullStr	Changes in Abundance of Oral Microbiota Associated with Oral Cancer
title_full_unstemmed	Changes in Abundance of Oral Microbiota Associated with Oral Cancer
title_sort	changes in abundance of oral microbiota associated with oral cancer
description	Individual bacteria and shifts in the composition of the microbiome have been associated with human diseases including cancer. To investigate changes in the microbiome associated with oral cancers, we profiled cancers and anatomically matched contralateral normal tissue from the same patient by sequencing 16S rDNA hypervariable region amplicons. In cancer samples from both a discovery and a subsequent confirmation cohort, abundance of Firmicutes (especially Streptococcus) and Actinobacteria (especially Rothia) was significantly decreased relative to contralateral normal samples from the same patient. Significant decreases in abundance of these phyla were observed for pre-cancers, but not when comparing samples from contralateral sites (tongue and floor of mouth) from healthy individuals. Weighted UniFrac principal coordinates analysis based on 12 taxa separated most cancers from other samples with greatest separation of node positive cases. These studies begin to develop a framework for exploiting the oral microbiome for monitoring oral cancer development, progression and recurrence.
publisher	Public Library of Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041887/
_version_	1612095489938817024

Changes in Abundance of Oral Microbiota Associated with Oral Cancer

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