Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro

Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro

Radiation-induced bystander effect (RIBE) has been defined as radiation responses observed in nonirradiated cells. It has been the focus of investigators worldwide due to the deleterious effects it induces in nonirradiated cells. The present study was performed to investigate whether acute or fracti...

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Main Authors: Soleymanifard, Shokouhozaman, Toossi, Mohammad Taghi Bahreyni, Samani, Roghayeh Kamran, Mohebbi, Shokoufeh
Format: Online
Language:English
Published: Medknow Publications & Media Pvt Ltd 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4035621/
id pubmed-4035621
recordtype oai_dc
spelling pubmed-40356212014-05-28 Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro Soleymanifard, Shokouhozaman Toossi, Mohammad Taghi Bahreyni Samani, Roghayeh Kamran Mohebbi, Shokoufeh Original Article Radiation-induced bystander effect (RIBE) has been defined as radiation responses observed in nonirradiated cells. It has been the focus of investigators worldwide due to the deleterious effects it induces in nonirradiated cells. The present study was performed to investigate whether acute or fractionated irradiation will evoke a differential bystander response in MRC5 cells. A normal human cell line (MRC5), and a human lung tumor cell line (QU-DB) were exposed to 0, 1, 2, and 4Gy of single acute or fractionated irradiation of equal fractions with a gap of 6 h. The MRC5 cells were supplemented with the media of irradiated cells and their micronucleus frequency was determined. The micronucleus frequency after single and fractionated irradiation did not vary significantly in the MRC5 cells conditioned with autologous or QU-DB cell-irradiated media, except for 4Gy where the frequency of micronucleated cells was lower in those MRC5 cells cultured in the media of QU-DB-exposed with a single dose of 4Gy. Our study demonstrates that the radiation-induced bystander effect was almost similar after single acute and fractionated exposure in MRC5 cells. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4035621/ /pubmed/24872606 http://dx.doi.org/10.4103/0971-6203.131282 Text en Copyright: © Journal of Medical Physics http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Soleymanifard, Shokouhozaman
Toossi, Mohammad Taghi Bahreyni
Samani, Roghayeh Kamran
Mohebbi, Shokoufeh
spellingShingle Soleymanifard, Shokouhozaman
Toossi, Mohammad Taghi Bahreyni
Samani, Roghayeh Kamran
Mohebbi, Shokoufeh
Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro
author_facet Soleymanifard, Shokouhozaman
Toossi, Mohammad Taghi Bahreyni
Samani, Roghayeh Kamran
Mohebbi, Shokoufeh
author_sort Soleymanifard, Shokouhozaman
title Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro
title_short Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro
title_full Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro
title_fullStr Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro
title_full_unstemmed Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro
title_sort investigation of the bystander effect in mrc5 cells after acute and fractionated irradiation in vitro
description Radiation-induced bystander effect (RIBE) has been defined as radiation responses observed in nonirradiated cells. It has been the focus of investigators worldwide due to the deleterious effects it induces in nonirradiated cells. The present study was performed to investigate whether acute or fractionated irradiation will evoke a differential bystander response in MRC5 cells. A normal human cell line (MRC5), and a human lung tumor cell line (QU-DB) were exposed to 0, 1, 2, and 4Gy of single acute or fractionated irradiation of equal fractions with a gap of 6 h. The MRC5 cells were supplemented with the media of irradiated cells and their micronucleus frequency was determined. The micronucleus frequency after single and fractionated irradiation did not vary significantly in the MRC5 cells conditioned with autologous or QU-DB cell-irradiated media, except for 4Gy where the frequency of micronucleated cells was lower in those MRC5 cells cultured in the media of QU-DB-exposed with a single dose of 4Gy. Our study demonstrates that the radiation-induced bystander effect was almost similar after single acute and fractionated exposure in MRC5 cells.
publisher Medknow Publications & Media Pvt Ltd
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4035621/
_version_ 1612093833296740352

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