DNA polymerase hybrids derived from the family-B enzymes of Pyrococcus furiosus and Thermococcus kodakarensis: improving performance in the polymerase chain reaction

DNA polymerase hybrids derived from the family-B enzymes of Pyrococcus furiosus and Thermococcus kodakarensis: improving performance in the polymerase chain reaction

The polymerase chain reaction (PCR) is widely applied across the biosciences, with archaeal Family-B DNA polymerases being preferred, due to their high thermostability and fidelity. The enzyme from Pyrococcus furiosus (Pfu-Pol) is more frequently used than the similar protein from Thermococcus kodak...

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Bibliographic Details
Main Authors: Elshawadfy, Ashraf M., Keith, Brian J., Ee Ooi, H'Ng, Kinsman, Thomas, Heslop, Pauline, Connolly, Bernard A.
Format: Online
Language:English
Published: Frontiers Media S.A. 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034419/

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034419/

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