Altered Oral Viral Ecology in Association with Periodontal Disease

The human oral cavity is home to a large and diverse community of viruses that have yet to be characterized in patients with periodontal disease. We recruited and sampled saliva and oral biofilm from a cohort of humans either periodontally healthy or with mild or significant periodontal disease to d...

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Main Authors:	Ly, Melissa, Abeles, Shira R., Boehm, Tobias K., Robles-Sikisaka, Refugio, Naidu, Mayuri, Santiago-Rodriguez, Tasha, Pride, David T.
Format:	Online
Language:	English
Published:	American Society of Microbiology 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030452/

id	pubmed-4030452
recordtype	oai_dc
spelling	pubmed-40304522014-06-06 Altered Oral Viral Ecology in Association with Periodontal Disease Ly, Melissa Abeles, Shira R. Boehm, Tobias K. Robles-Sikisaka, Refugio Naidu, Mayuri Santiago-Rodriguez, Tasha Pride, David T. Research Article The human oral cavity is home to a large and diverse community of viruses that have yet to be characterized in patients with periodontal disease. We recruited and sampled saliva and oral biofilm from a cohort of humans either periodontally healthy or with mild or significant periodontal disease to discern whether there are differences in viral communities that reflect their oral health status. We found communities of viruses inhabiting saliva and the subgingival and supragingival biofilms of each subject that were composed largely of bacteriophage. While there were homologous viruses common to different subjects and biogeographic sites, for most of the subjects, virome compositions were significantly associated with the oral sites from which they were derived. The largest distinctions between virome compositions were found when comparing the subgingival and supragingival biofilms to those of planktonic saliva. Differences in virome composition were significantly associated with oral health status for both subgingival and supragingival biofilm viruses but not for salivary viruses. Among the differences identified in virome compositions was a significant expansion of myoviruses in subgingival biofilm, suggesting that periodontal disease favors lytic phage. We also characterized the bacterial communities in each subject at each biogeographic site by using the V3 hypervariable segment of the 16S rRNA and did not identify distinctions between oral health and disease similar to those found in viral communities. The significantly altered ecology of viruses of oral biofilm in subjects with periodontal disease compared to that of relatively periodontally healthy ones suggests that viruses may serve as useful indicators of oral health status. American Society of Microbiology 2014-05-20 /pmc/articles/PMC4030452/ /pubmed/24846382 http://dx.doi.org/10.1128/mBio.01133-14 Text en Copyright © 2014 Ly et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ly, Melissa Abeles, Shira R. Boehm, Tobias K. Robles-Sikisaka, Refugio Naidu, Mayuri Santiago-Rodriguez, Tasha Pride, David T.
spellingShingle	Ly, Melissa Abeles, Shira R. Boehm, Tobias K. Robles-Sikisaka, Refugio Naidu, Mayuri Santiago-Rodriguez, Tasha Pride, David T. Altered Oral Viral Ecology in Association with Periodontal Disease
author_facet	Ly, Melissa Abeles, Shira R. Boehm, Tobias K. Robles-Sikisaka, Refugio Naidu, Mayuri Santiago-Rodriguez, Tasha Pride, David T.
author_sort	Ly, Melissa
title	Altered Oral Viral Ecology in Association with Periodontal Disease
title_short	Altered Oral Viral Ecology in Association with Periodontal Disease
title_full	Altered Oral Viral Ecology in Association with Periodontal Disease
title_fullStr	Altered Oral Viral Ecology in Association with Periodontal Disease
title_full_unstemmed	Altered Oral Viral Ecology in Association with Periodontal Disease
title_sort	altered oral viral ecology in association with periodontal disease
description	The human oral cavity is home to a large and diverse community of viruses that have yet to be characterized in patients with periodontal disease. We recruited and sampled saliva and oral biofilm from a cohort of humans either periodontally healthy or with mild or significant periodontal disease to discern whether there are differences in viral communities that reflect their oral health status. We found communities of viruses inhabiting saliva and the subgingival and supragingival biofilms of each subject that were composed largely of bacteriophage. While there were homologous viruses common to different subjects and biogeographic sites, for most of the subjects, virome compositions were significantly associated with the oral sites from which they were derived. The largest distinctions between virome compositions were found when comparing the subgingival and supragingival biofilms to those of planktonic saliva. Differences in virome composition were significantly associated with oral health status for both subgingival and supragingival biofilm viruses but not for salivary viruses. Among the differences identified in virome compositions was a significant expansion of myoviruses in subgingival biofilm, suggesting that periodontal disease favors lytic phage. We also characterized the bacterial communities in each subject at each biogeographic site by using the V3 hypervariable segment of the 16S rRNA and did not identify distinctions between oral health and disease similar to those found in viral communities. The significantly altered ecology of viruses of oral biofilm in subjects with periodontal disease compared to that of relatively periodontally healthy ones suggests that viruses may serve as useful indicators of oral health status.
publisher	American Society of Microbiology
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030452/
_version_	1612092129283145728

Altered Oral Viral Ecology in Association with Periodontal Disease

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