The piggyBac Transposon-Mediated Expression of SV40 T Antigen Efficiently Immortalizes Mouse Embryonic Fibroblasts (MEFs)

Mouse embryonic fibroblasts (MEFs) are mesenchymal stem cell (MSC)-like multipotent progenitor cells and can undergo self-renewal and differentiate into to multiple lineages, including bone, cartilage and adipose. Primary MEFs have limited life span in culture, which thus hampers MEFs’ basic researc...

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Bibliographic Details
Main Authors: Wang, Ning, Zhang, Wenwen, Cui, Jing, Zhang, Hongmei, Chen, Xiang, Li, Ruidong, Wu, Ningning, Chen, Xian, Wen, Sheng, Zhang, Junhui, Yin, Liangjun, Deng, Fang, Liao, Zhan, Zhang, Zhonglin, Zhang, Qian, Yan, Zhengjian, Liu, Wei, Ye, Jixing, Deng, Youlin, Wang, Zhongliang, Qiao, Min, Luu, Hue H., Haydon, Rex C., Shi, Lewis L., Liang, Houjie, He, Tong-Chuan
Format: Online
Language:English
Published: Public Library of Science 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4028212/