The piggyBac Transposon-Mediated Expression of SV40 T Antigen Efficiently Immortalizes Mouse Embryonic Fibroblasts (MEFs)
Mouse embryonic fibroblasts (MEFs) are mesenchymal stem cell (MSC)-like multipotent progenitor cells and can undergo self-renewal and differentiate into to multiple lineages, including bone, cartilage and adipose. Primary MEFs have limited life span in culture, which thus hampers MEFs’ basic researc...
Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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Format: | Online |
Language: | English |
Published: |
Public Library of Science
2014
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4028212/ |