A synthetic luciferin improves bioluminescence imaging in live mice

Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice wi...

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Main Authors:	Evans, Melanie S., Chaurette, Joanna P., Adams, Spencer T., Reddy, Gadarla R., Paley, Miranda A., Aronin, Neil, Prescher, Jennifer A., Miller, Stephen C.
Format:	Online
Language:	English
Published:	2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4026177/

id	pubmed-4026177
recordtype	oai_dc
spelling	pubmed-40261772014-10-01 A synthetic luciferin improves bioluminescence imaging in live mice Evans, Melanie S. Chaurette, Joanna P. Adams, Spencer T. Reddy, Gadarla R. Paley, Miranda A. Aronin, Neil Prescher, Jennifer A. Miller, Stephen C. Article Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI from existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin. 2014-02-09 2014-04 /pmc/articles/PMC4026177/ /pubmed/24509630 http://dx.doi.org/10.1038/nmeth.2839 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Evans, Melanie S. Chaurette, Joanna P. Adams, Spencer T. Reddy, Gadarla R. Paley, Miranda A. Aronin, Neil Prescher, Jennifer A. Miller, Stephen C.
spellingShingle	Evans, Melanie S. Chaurette, Joanna P. Adams, Spencer T. Reddy, Gadarla R. Paley, Miranda A. Aronin, Neil Prescher, Jennifer A. Miller, Stephen C. A synthetic luciferin improves bioluminescence imaging in live mice
author_facet	Evans, Melanie S. Chaurette, Joanna P. Adams, Spencer T. Reddy, Gadarla R. Paley, Miranda A. Aronin, Neil Prescher, Jennifer A. Miller, Stephen C.
author_sort	Evans, Melanie S.
title	A synthetic luciferin improves bioluminescence imaging in live mice
title_short	A synthetic luciferin improves bioluminescence imaging in live mice
title_full	A synthetic luciferin improves bioluminescence imaging in live mice
title_fullStr	A synthetic luciferin improves bioluminescence imaging in live mice
title_full_unstemmed	A synthetic luciferin improves bioluminescence imaging in live mice
title_sort	synthetic luciferin improves bioluminescence imaging in live mice
description	Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI from existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4026177/
_version_	1612090669480804352

A synthetic luciferin improves bioluminescence imaging in live mice

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