Significant Productivity Improvement of the Baculovirus Expression Vector System by Engineering a Novel Expression Cassette

Here we describe the development of a baculovirus vector expression cassette containing rearranged baculovirus-derived genetic regulatory elements. This newly designed expression cassette conferred significant production improvements to the baculovirus expression vector system (BEVS), including prol...

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Main Authors:	Gómez-Sebastián, Silvia, López-Vidal, Javier, Escribano, José M.
Format:	Online
Language:	English
Published:	Public Library of Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019511/

id	pubmed-4019511
recordtype	oai_dc
spelling	pubmed-40195112014-05-16 Significant Productivity Improvement of the Baculovirus Expression Vector System by Engineering a Novel Expression Cassette Gómez-Sebastián, Silvia López-Vidal, Javier Escribano, José M. Research Article Here we describe the development of a baculovirus vector expression cassette containing rearranged baculovirus-derived genetic regulatory elements. This newly designed expression cassette conferred significant production improvements to the baculovirus expression vector system (BEVS), including prolonged cell integrity after infection, improved protein integrity, and around 4-fold increase in recombinant protein production yields in insect cells with respect to a standard baculovirus vector. The expression cassette consisted of a cDNA encoding for the baculovirus transactivation factors IE1 and IE0, expressed under the control of the polyhedrin promoter, and a homologous repeated transcription enhancer sequence operatively cis-linked to the p10 promoter or to chimeric promoters containing p10. The prolonged cell integrity observed in cells infected by baculoviruses harbouring the novel expression cassette reduced the characteristic proteolysis and aberrant forms frequently found in baculovirus-derived recombinant proteins. The new expression cassette developed here has the potential to significantly improve the productivity of the BEVS. Public Library of Science 2014-05-13 /pmc/articles/PMC4019511/ /pubmed/24824596 http://dx.doi.org/10.1371/journal.pone.0096562 Text en © 2014 Gómez-Sebastián et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Gómez-Sebastián, Silvia López-Vidal, Javier Escribano, José M.
spellingShingle	Gómez-Sebastián, Silvia López-Vidal, Javier Escribano, José M. Significant Productivity Improvement of the Baculovirus Expression Vector System by Engineering a Novel Expression Cassette
author_facet	Gómez-Sebastián, Silvia López-Vidal, Javier Escribano, José M.
author_sort	Gómez-Sebastián, Silvia
title	Significant Productivity Improvement of the Baculovirus Expression Vector System by Engineering a Novel Expression Cassette
title_short	Significant Productivity Improvement of the Baculovirus Expression Vector System by Engineering a Novel Expression Cassette
title_full	Significant Productivity Improvement of the Baculovirus Expression Vector System by Engineering a Novel Expression Cassette
title_fullStr	Significant Productivity Improvement of the Baculovirus Expression Vector System by Engineering a Novel Expression Cassette
title_full_unstemmed	Significant Productivity Improvement of the Baculovirus Expression Vector System by Engineering a Novel Expression Cassette
title_sort	significant productivity improvement of the baculovirus expression vector system by engineering a novel expression cassette
description	Here we describe the development of a baculovirus vector expression cassette containing rearranged baculovirus-derived genetic regulatory elements. This newly designed expression cassette conferred significant production improvements to the baculovirus expression vector system (BEVS), including prolonged cell integrity after infection, improved protein integrity, and around 4-fold increase in recombinant protein production yields in insect cells with respect to a standard baculovirus vector. The expression cassette consisted of a cDNA encoding for the baculovirus transactivation factors IE1 and IE0, expressed under the control of the polyhedrin promoter, and a homologous repeated transcription enhancer sequence operatively cis-linked to the p10 promoter or to chimeric promoters containing p10. The prolonged cell integrity observed in cells infected by baculoviruses harbouring the novel expression cassette reduced the characteristic proteolysis and aberrant forms frequently found in baculovirus-derived recombinant proteins. The new expression cassette developed here has the potential to significantly improve the productivity of the BEVS.
publisher	Public Library of Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019511/
_version_	1612088441648971776

Significant Productivity Improvement of the Baculovirus Expression Vector System by Engineering a Novel Expression Cassette

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