Using 15N-Ammonium to Characterise and Map Potassium Binding Sites in Proteins by NMR Spectroscopy
A variety of enzymes are activated by the binding of potassium ions. The potassium binding sites of these enzymes are very specific, but ammonium ions can often replace potassium ions in vitro because of their similar ionic radii. In these cases, ammonium can be used as a proxy for potassium to char...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | English |
| Published: |
WILEY-VCH Verlag
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016748/ |