Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC

Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC

A reversed-phase high-performance liquid chromatographic method has been developed to quantitatively determine matrine in liquid crystal nanoparticles. The chromatographic method is carried out using an isocratic system. The mobile phase was composed of methanol-PBS(pH6.8)-triethylamine (50 : 50 : 0...

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Main Authors: Peng, Xinsheng, Li, Baohong, Hu, Min, Ling, Yahao, Tian, Yuan, Zhou, Yanxing, Zhou, Yanfang
Format: Online
Language:English
Published: Hindawi Publishing Corporation 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009329/
id pubmed-4009329
recordtype oai_dc
spelling pubmed-40093292014-05-15 Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC Peng, Xinsheng Li, Baohong Hu, Min Ling, Yahao Tian, Yuan Zhou, Yanxing Zhou, Yanfang Research Article A reversed-phase high-performance liquid chromatographic method has been developed to quantitatively determine matrine in liquid crystal nanoparticles. The chromatographic method is carried out using an isocratic system. The mobile phase was composed of methanol-PBS(pH6.8)-triethylamine (50 : 50 : 0.1%) with a flow rate of 1 mL/min with SPD-20A UV/vis detector and the detection wavelength was at 220 nm. The linearity of matrine is in the range of 1.6 to 200.0 μg/mL. The regression equation is y = 10706x − 2959 (R 2 = 1.0). The average recovery is 101.7%; RSD = 2.22%  (n = 9). This method provides a simple and accurate strategy to determine matrine in liquid crystalline nanoparticle. Hindawi Publishing Corporation 2014 2014-04-14 /pmc/articles/PMC4009329/ /pubmed/24834359 http://dx.doi.org/10.1155/2014/368682 Text en Copyright © 2014 Xinsheng Peng et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Peng, Xinsheng
Li, Baohong
Hu, Min
Ling, Yahao
Tian, Yuan
Zhou, Yanxing
Zhou, Yanfang
spellingShingle Peng, Xinsheng
Li, Baohong
Hu, Min
Ling, Yahao
Tian, Yuan
Zhou, Yanxing
Zhou, Yanfang
Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
author_facet Peng, Xinsheng
Li, Baohong
Hu, Min
Ling, Yahao
Tian, Yuan
Zhou, Yanxing
Zhou, Yanfang
author_sort Peng, Xinsheng
title Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
title_short Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
title_full Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
title_fullStr Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
title_full_unstemmed Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
title_sort quantitative analysis of matrine in liquid crystalline nanoparticles by hplc
description A reversed-phase high-performance liquid chromatographic method has been developed to quantitatively determine matrine in liquid crystal nanoparticles. The chromatographic method is carried out using an isocratic system. The mobile phase was composed of methanol-PBS(pH6.8)-triethylamine (50 : 50 : 0.1%) with a flow rate of 1 mL/min with SPD-20A UV/vis detector and the detection wavelength was at 220 nm. The linearity of matrine is in the range of 1.6 to 200.0 μg/mL. The regression equation is y = 10706x − 2959 (R 2 = 1.0). The average recovery is 101.7%; RSD = 2.22%  (n = 9). This method provides a simple and accurate strategy to determine matrine in liquid crystalline nanoparticle.
publisher Hindawi Publishing Corporation
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009329/
_version_ 1612085164794445824

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