KRAS and BRAF genotyping of synchronous colorectal carcinomas

v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) genotyping is required prior to anti-epidermal growth factor receptor monoclonal antibody therapy administered in cases of metastatic colorectal carcinoma (CRC). Thus, KRAS mutation screening is required for patient management. The present...

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Main Authors:	GIANNINI, RICCARDO, LUPI, CRISTIANA, LOUPAKIS, FOTIOS, SERVADIO, ADELE, CREMOLINI, CHIARA, SENSI, ELISA, CHIARUGI, MASSIMO, ANTONIOTTI, CARLOTTA, BASOLO, FULVIO, FALCONE, ALFREDO, FONTANINI, GABRIELLA
Format:	Online
Language:	English
Published:	D.A. Spandidos 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997682/

id	pubmed-3997682
recordtype	oai_dc
spelling	pubmed-39976822014-04-24 KRAS and BRAF genotyping of synchronous colorectal carcinomas GIANNINI, RICCARDO LUPI, CRISTIANA LOUPAKIS, FOTIOS SERVADIO, ADELE CREMOLINI, CHIARA SENSI, ELISA CHIARUGI, MASSIMO ANTONIOTTI, CARLOTTA BASOLO, FULVIO FALCONE, ALFREDO FONTANINI, GABRIELLA Articles v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) genotyping is required prior to anti-epidermal growth factor receptor monoclonal antibody therapy administered in cases of metastatic colorectal carcinoma (CRC). Thus, KRAS mutation screening is required for patient management. The present study reported the experience of KRAS/v-raf murine sarcoma viral oncogene homolog B1 (BRAF) mutational screening on synchronous CRC pairs from 26 patients, which were defined as index lesions (ILs) and concurrent lesions (CLs) on the basis of tumor grade and dimension and their respective lymph node and distant metastases. Overall, KRAS mutations were present in 38.4% of patients, whereas BRAF mutations were present at a frequency of 11.5%. The genotyping of paired synchronous carcinomas indicated that 11 patients (42.3%) exhibited discordant KRAS mutational statuses in terms of the presence of a mutation in only one lesion of the pair or of two different mutations harbored by each lesion. BRAF mutations were present in the synchronous tumors of two cases, whereas in two other cases, only the IL or CL harbored mutant BRAF. Overall, the mutational statuses of distant and lymph node metastases confirm the genetic heterogeneity of synchronous primary tumors. These results highlighted the fact that adequate sampling and comprehensive testing, when feasible, is likely to optimize the decision-making process for treatment approaches, even in the relatively rare event of multiple synchronous lesions. D.A. Spandidos 2014-05 2014-02-21 /pmc/articles/PMC3997682/ /pubmed/24765171 http://dx.doi.org/10.3892/ol.2014.1905 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	GIANNINI, RICCARDO LUPI, CRISTIANA LOUPAKIS, FOTIOS SERVADIO, ADELE CREMOLINI, CHIARA SENSI, ELISA CHIARUGI, MASSIMO ANTONIOTTI, CARLOTTA BASOLO, FULVIO FALCONE, ALFREDO FONTANINI, GABRIELLA
spellingShingle	GIANNINI, RICCARDO LUPI, CRISTIANA LOUPAKIS, FOTIOS SERVADIO, ADELE CREMOLINI, CHIARA SENSI, ELISA CHIARUGI, MASSIMO ANTONIOTTI, CARLOTTA BASOLO, FULVIO FALCONE, ALFREDO FONTANINI, GABRIELLA KRAS and BRAF genotyping of synchronous colorectal carcinomas
author_facet	GIANNINI, RICCARDO LUPI, CRISTIANA LOUPAKIS, FOTIOS SERVADIO, ADELE CREMOLINI, CHIARA SENSI, ELISA CHIARUGI, MASSIMO ANTONIOTTI, CARLOTTA BASOLO, FULVIO FALCONE, ALFREDO FONTANINI, GABRIELLA
author_sort	GIANNINI, RICCARDO
title	KRAS and BRAF genotyping of synchronous colorectal carcinomas
title_short	KRAS and BRAF genotyping of synchronous colorectal carcinomas
title_full	KRAS and BRAF genotyping of synchronous colorectal carcinomas
title_fullStr	KRAS and BRAF genotyping of synchronous colorectal carcinomas
title_full_unstemmed	KRAS and BRAF genotyping of synchronous colorectal carcinomas
title_sort	kras and braf genotyping of synchronous colorectal carcinomas
description	v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) genotyping is required prior to anti-epidermal growth factor receptor monoclonal antibody therapy administered in cases of metastatic colorectal carcinoma (CRC). Thus, KRAS mutation screening is required for patient management. The present study reported the experience of KRAS/v-raf murine sarcoma viral oncogene homolog B1 (BRAF) mutational screening on synchronous CRC pairs from 26 patients, which were defined as index lesions (ILs) and concurrent lesions (CLs) on the basis of tumor grade and dimension and their respective lymph node and distant metastases. Overall, KRAS mutations were present in 38.4% of patients, whereas BRAF mutations were present at a frequency of 11.5%. The genotyping of paired synchronous carcinomas indicated that 11 patients (42.3%) exhibited discordant KRAS mutational statuses in terms of the presence of a mutation in only one lesion of the pair or of two different mutations harbored by each lesion. BRAF mutations were present in the synchronous tumors of two cases, whereas in two other cases, only the IL or CL harbored mutant BRAF. Overall, the mutational statuses of distant and lymph node metastases confirm the genetic heterogeneity of synchronous primary tumors. These results highlighted the fact that adequate sampling and comprehensive testing, when feasible, is likely to optimize the decision-making process for treatment approaches, even in the relatively rare event of multiple synchronous lesions.
publisher	D.A. Spandidos
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997682/
_version_	1612081678160756736

KRAS and BRAF genotyping of synchronous colorectal carcinomas

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