Combinatorial scaffold morphologies for zonal articular cartilage engineering☆

Articular cartilage lesions are a particular challenge for regenerative medicine strategies as cartilage function stems from a complex depth-dependent organization. Tissue engineering scaffolds that vary in morphology and function offer a template for zone-specific cartilage extracellular matrix (EC...

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Main Authors:	Steele, J.A.M., McCullen, S.D., Callanan, A., Autefage, H., Accardi, M.A., Dini, D., Stevens, M.M.
Format:	Online
Language:	English
Published:	Elsevier 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991416/

id	pubmed-3991416
recordtype	oai_dc
spelling	pubmed-39914162014-05-01 Combinatorial scaffold morphologies for zonal articular cartilage engineering☆ Steele, J.A.M. McCullen, S.D. Callanan, A. Autefage, H. Accardi, M.A. Dini, D. Stevens, M.M. Article Articular cartilage lesions are a particular challenge for regenerative medicine strategies as cartilage function stems from a complex depth-dependent organization. Tissue engineering scaffolds that vary in morphology and function offer a template for zone-specific cartilage extracellular matrix (ECM) production and mechanical properties. We fabricated multi-zone cartilage scaffolds by the electrostatic deposition of polymer microfibres onto particulate-templated scaffolds produced with 0.03 or 1.0 mm3 porogens. The scaffolds allowed ample space for chondrocyte ECM production within the bulk while also mimicking the structural organization and functional interface of cartilage’s superficial zone. Addition of aligned fibre membranes enhanced the mechanical and surface properties of particulate-templated scaffolds. Zonal analysis of scaffolds demonstrated region-specific variations in chondrocyte number, sulfated GAG-rich ECM, and chondrocytic gene expression. Specifically, smaller porogens (0.03 mm3) yielded significantly higher sGAG accumulation and aggrecan gene expression. Our results demonstrate that bilayered scaffolds mimic some key structural characteristics of native cartilage, support in vitro cartilage formation, and have superior features to homogeneous particulate-templated scaffolds. We propose that these scaffolds offer promise for regenerative medicine strategies to repair articular cartilage lesions. Elsevier 2014-05 /pmc/articles/PMC3991416/ /pubmed/24370641 http://dx.doi.org/10.1016/j.actbio.2013.12.030 Text en © 2013 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Steele, J.A.M. McCullen, S.D. Callanan, A. Autefage, H. Accardi, M.A. Dini, D. Stevens, M.M.
spellingShingle	Steele, J.A.M. McCullen, S.D. Callanan, A. Autefage, H. Accardi, M.A. Dini, D. Stevens, M.M. Combinatorial scaffold morphologies for zonal articular cartilage engineering☆
author_facet	Steele, J.A.M. McCullen, S.D. Callanan, A. Autefage, H. Accardi, M.A. Dini, D. Stevens, M.M.
author_sort	Steele, J.A.M.
title	Combinatorial scaffold morphologies for zonal articular cartilage engineering☆
title_short	Combinatorial scaffold morphologies for zonal articular cartilage engineering☆
title_full	Combinatorial scaffold morphologies for zonal articular cartilage engineering☆
title_fullStr	Combinatorial scaffold morphologies for zonal articular cartilage engineering☆
title_full_unstemmed	Combinatorial scaffold morphologies for zonal articular cartilage engineering☆
title_sort	combinatorial scaffold morphologies for zonal articular cartilage engineering☆
description	Articular cartilage lesions are a particular challenge for regenerative medicine strategies as cartilage function stems from a complex depth-dependent organization. Tissue engineering scaffolds that vary in morphology and function offer a template for zone-specific cartilage extracellular matrix (ECM) production and mechanical properties. We fabricated multi-zone cartilage scaffolds by the electrostatic deposition of polymer microfibres onto particulate-templated scaffolds produced with 0.03 or 1.0 mm3 porogens. The scaffolds allowed ample space for chondrocyte ECM production within the bulk while also mimicking the structural organization and functional interface of cartilage’s superficial zone. Addition of aligned fibre membranes enhanced the mechanical and surface properties of particulate-templated scaffolds. Zonal analysis of scaffolds demonstrated region-specific variations in chondrocyte number, sulfated GAG-rich ECM, and chondrocytic gene expression. Specifically, smaller porogens (0.03 mm3) yielded significantly higher sGAG accumulation and aggrecan gene expression. Our results demonstrate that bilayered scaffolds mimic some key structural characteristics of native cartilage, support in vitro cartilage formation, and have superior features to homogeneous particulate-templated scaffolds. We propose that these scaffolds offer promise for regenerative medicine strategies to repair articular cartilage lesions.
publisher	Elsevier
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991416/
_version_	1612079741380067328

Combinatorial scaffold morphologies for zonal articular cartilage engineering☆

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