Formation of Sclerotia and Production of Indoloterpenes by Aspergillus niger and Other Species in Section Nigri

Several species in Aspergillus section Nigri have been reported to produce sclerotia on well-known growth media, such as Czapek yeast autolysate (CYA) agar, with sclerotia considered to be an important prerequisite for sexual development. However Aspergillus niger sensu stricto has not been reported...

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Main Authors:	Frisvad, Jens C., Petersen, Lene M., Lyhne, E. Kirstine, Larsen, Thomas O.
Format:	Online
Language:	English
Published:	Public Library of Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988082/

id	pubmed-3988082
recordtype	oai_dc
spelling	pubmed-39880822014-04-21 Formation of Sclerotia and Production of Indoloterpenes by Aspergillus niger and Other Species in Section Nigri Frisvad, Jens C. Petersen, Lene M. Lyhne, E. Kirstine Larsen, Thomas O. Research Article Several species in Aspergillus section Nigri have been reported to produce sclerotia on well-known growth media, such as Czapek yeast autolysate (CYA) agar, with sclerotia considered to be an important prerequisite for sexual development. However Aspergillus niger sensu stricto has not been reported to produce sclerotia, and is thought to be a purely asexual organism. Here we report, for the first time, the production of sclerotia by certain strains of Aspergillus niger when grown on CYA agar with raisins, or on other fruits or on rice. Up to 11 apolar indoloterpenes of the aflavinine type were detected by liquid chromatography and diode array and mass spectrometric detection where sclerotia were formed, including 10,23-dihydro-24,25-dehydroaflavinine. Sclerotium induction can thus be a way of inducing the production of new secondary metabolites from previously silent gene clusters. Cultivation of other species of the black aspergilli showed that raisins induced sclerotium formation by A. brasiliensis, A. floridensis A. ibericus, A. luchuensis, A. neoniger, A. trinidadensis and A. saccharolyticus for the first time. Public Library of Science 2014-04-15 /pmc/articles/PMC3988082/ /pubmed/24736731 http://dx.doi.org/10.1371/journal.pone.0094857 Text en © 2014 Frisvad et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Frisvad, Jens C. Petersen, Lene M. Lyhne, E. Kirstine Larsen, Thomas O.
spellingShingle	Frisvad, Jens C. Petersen, Lene M. Lyhne, E. Kirstine Larsen, Thomas O. Formation of Sclerotia and Production of Indoloterpenes by Aspergillus niger and Other Species in Section Nigri
author_facet	Frisvad, Jens C. Petersen, Lene M. Lyhne, E. Kirstine Larsen, Thomas O.
author_sort	Frisvad, Jens C.
title	Formation of Sclerotia and Production of Indoloterpenes by Aspergillus niger and Other Species in Section Nigri
title_short	Formation of Sclerotia and Production of Indoloterpenes by Aspergillus niger and Other Species in Section Nigri
title_full	Formation of Sclerotia and Production of Indoloterpenes by Aspergillus niger and Other Species in Section Nigri
title_fullStr	Formation of Sclerotia and Production of Indoloterpenes by Aspergillus niger and Other Species in Section Nigri
title_full_unstemmed	Formation of Sclerotia and Production of Indoloterpenes by Aspergillus niger and Other Species in Section Nigri
title_sort	formation of sclerotia and production of indoloterpenes by aspergillus niger and other species in section nigri
description	Several species in Aspergillus section Nigri have been reported to produce sclerotia on well-known growth media, such as Czapek yeast autolysate (CYA) agar, with sclerotia considered to be an important prerequisite for sexual development. However Aspergillus niger sensu stricto has not been reported to produce sclerotia, and is thought to be a purely asexual organism. Here we report, for the first time, the production of sclerotia by certain strains of Aspergillus niger when grown on CYA agar with raisins, or on other fruits or on rice. Up to 11 apolar indoloterpenes of the aflavinine type were detected by liquid chromatography and diode array and mass spectrometric detection where sclerotia were formed, including 10,23-dihydro-24,25-dehydroaflavinine. Sclerotium induction can thus be a way of inducing the production of new secondary metabolites from previously silent gene clusters. Cultivation of other species of the black aspergilli showed that raisins induced sclerotium formation by A. brasiliensis, A. floridensis A. ibericus, A. luchuensis, A. neoniger, A. trinidadensis and A. saccharolyticus for the first time.
publisher	Public Library of Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988082/
_version_	1612078607158476800

Formation of Sclerotia and Production of Indoloterpenes by Aspergillus niger and Other Species in Section Nigri

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