Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog

The aim of the present study was to report a novel developmental abnormality in a cloned dog. A fibroblast cell line was established from an 8-year-old male German shepherd dog. In vivo matured oocytes were retrieved from a large breed dog, and the nucleus was removed from each oocyte. A donor cell...

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Main Authors:	Kim, Min Jung, Oh, Hyun Ju, Kim, Geon A, Jo, Young Kwang, Choi, Jin, Kim, Hye Jin, Choi, Hee Yeon, Kim, Hyun Wook, Choi, Min Cheol, Lee, Byeong Chun
Format:	Online
Language:	English
Published:	BioMed Central 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984017/

id	pubmed-3984017
recordtype	oai_dc
spelling	pubmed-39840172014-04-12 Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog Kim, Min Jung Oh, Hyun Ju Kim, Geon A Jo, Young Kwang Choi, Jin Kim, Hye Jin Choi, Hee Yeon Kim, Hyun Wook Choi, Min Cheol Lee, Byeong Chun Case Report The aim of the present study was to report a novel developmental abnormality in a cloned dog. A fibroblast cell line was established from an 8-year-old male German shepherd dog. In vivo matured oocytes were retrieved from a large breed dog, and the nucleus was removed from each oocyte. A donor cell was injected into an enucleated oocyte, and the oocyte-cell couplet was fused electrically. After chemical activation, the resulting embryos were transferred into a naturally estrus-synchronized recipient dog, and two cloned pups were delivered by Cesarean section 60 days later. One cloned pup (Clone 1) was healthy, but the other (Clone 2) had a birth weight of only 320 g and cleft palate, failure of preputial closure at the ventral distal part, and persistent penile frenulum. Clone 2 was raised by stomach feeding until Day 40 after birth, where palatoplasty was performed. The abnormalities in external genitalia in Clone 2 resulted in persistent penile extrusion that was surgically corrected. This complex developmental abnormality has not been reported in dogs previously. BioMed Central 2014-03-26 /pmc/articles/PMC3984017/ /pubmed/24669802 http://dx.doi.org/10.1186/1751-0147-56-18 Text en Copyright © 2014 Kim et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kim, Min Jung Oh, Hyun Ju Kim, Geon A Jo, Young Kwang Choi, Jin Kim, Hye Jin Choi, Hee Yeon Kim, Hyun Wook Choi, Min Cheol Lee, Byeong Chun
spellingShingle	Kim, Min Jung Oh, Hyun Ju Kim, Geon A Jo, Young Kwang Choi, Jin Kim, Hye Jin Choi, Hee Yeon Kim, Hyun Wook Choi, Min Cheol Lee, Byeong Chun Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog
author_facet	Kim, Min Jung Oh, Hyun Ju Kim, Geon A Jo, Young Kwang Choi, Jin Kim, Hye Jin Choi, Hee Yeon Kim, Hyun Wook Choi, Min Cheol Lee, Byeong Chun
author_sort	Kim, Min Jung
title	Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog
title_short	Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog
title_full	Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog
title_fullStr	Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog
title_full_unstemmed	Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog
title_sort	reduced birth weight, cleft palate and preputial abnormalities in a cloned dog
description	The aim of the present study was to report a novel developmental abnormality in a cloned dog. A fibroblast cell line was established from an 8-year-old male German shepherd dog. In vivo matured oocytes were retrieved from a large breed dog, and the nucleus was removed from each oocyte. A donor cell was injected into an enucleated oocyte, and the oocyte-cell couplet was fused electrically. After chemical activation, the resulting embryos were transferred into a naturally estrus-synchronized recipient dog, and two cloned pups were delivered by Cesarean section 60 days later. One cloned pup (Clone 1) was healthy, but the other (Clone 2) had a birth weight of only 320 g and cleft palate, failure of preputial closure at the ventral distal part, and persistent penile frenulum. Clone 2 was raised by stomach feeding until Day 40 after birth, where palatoplasty was performed. The abnormalities in external genitalia in Clone 2 resulted in persistent penile extrusion that was surgically corrected. This complex developmental abnormality has not been reported in dogs previously.
publisher	BioMed Central
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984017/
_version_	1612077164134400000

Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog

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