Antigenic and genetic characterization of a divergent African virus, Ikoma lyssavirus
In 2009, a novel lyssavirus (subsequently named Ikoma lyssavirus, IKOV) was detected in the brain of an African civet (Civettictis civetta) with clinical rabies in the Serengeti National Park of Tanzania. The degree of nucleotide divergence between the genome of IKOV and those of other lyssaviruses...
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Society for General Microbiology
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983756/ |
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pubmed-39837562014-05-01 Antigenic and genetic characterization of a divergent African virus, Ikoma lyssavirus Horton, Daniel L. Banyard, Ashley C. Marston, Denise A. Wise, Emma Selden, David Nunez, Alejandro Hicks, Daniel Lembo, Tiziana Cleaveland, Sarah Peel, Alison J. Kuzmin, Ivan V. Rupprecht, Charles E. Fooks, Anthony R. Animal viruses In 2009, a novel lyssavirus (subsequently named Ikoma lyssavirus, IKOV) was detected in the brain of an African civet (Civettictis civetta) with clinical rabies in the Serengeti National Park of Tanzania. The degree of nucleotide divergence between the genome of IKOV and those of other lyssaviruses predicted antigenic distinction from, and lack of protection provided by, available rabies vaccines. In addition, the index case was considered likely to be an incidental spillover event, and therefore the true reservoir of IKOV remained to be identified. The advent of sensitive molecular techniques has led to a rapid increase in the discovery of novel viruses. Detecting viral sequence alone, however, only allows for prediction of phenotypic characteristics and not their measurement. In the present study we describe the in vitro and in vivo characterization of IKOV, demonstrating that it is (1) pathogenic by peripheral inoculation in an animal model, (2) antigenically distinct from current rabies vaccine strains and (3) poorly neutralized by sera from humans and animals immunized against rabies. In a laboratory mouse model, no protection was elicited by a licensed rabies vaccine. We also investigated the role of bats as reservoirs of IKOV. We found no evidence for infection among 483 individuals of at least 13 bat species sampled across sites in the Serengeti and Southern Kenya. Society for General Microbiology 2014-05 /pmc/articles/PMC3983756/ /pubmed/24496827 http://dx.doi.org/10.1099/vir.0.061952-0 Text en © 2014 Crown Copyright http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Horton, Daniel L. Banyard, Ashley C. Marston, Denise A. Wise, Emma Selden, David Nunez, Alejandro Hicks, Daniel Lembo, Tiziana Cleaveland, Sarah Peel, Alison J. Kuzmin, Ivan V. Rupprecht, Charles E. Fooks, Anthony R. |
| spellingShingle |
Horton, Daniel L. Banyard, Ashley C. Marston, Denise A. Wise, Emma Selden, David Nunez, Alejandro Hicks, Daniel Lembo, Tiziana Cleaveland, Sarah Peel, Alison J. Kuzmin, Ivan V. Rupprecht, Charles E. Fooks, Anthony R. Antigenic and genetic characterization of a divergent African virus, Ikoma lyssavirus |
| author_facet |
Horton, Daniel L. Banyard, Ashley C. Marston, Denise A. Wise, Emma Selden, David Nunez, Alejandro Hicks, Daniel Lembo, Tiziana Cleaveland, Sarah Peel, Alison J. Kuzmin, Ivan V. Rupprecht, Charles E. Fooks, Anthony R. |
| author_sort |
Horton, Daniel L. |
| title |
Antigenic and genetic characterization of a divergent African virus, Ikoma lyssavirus |
| title_short |
Antigenic and genetic characterization of a divergent African virus, Ikoma lyssavirus |
| title_full |
Antigenic and genetic characterization of a divergent African virus, Ikoma lyssavirus |
| title_fullStr |
Antigenic and genetic characterization of a divergent African virus, Ikoma lyssavirus |
| title_full_unstemmed |
Antigenic and genetic characterization of a divergent African virus, Ikoma lyssavirus |
| title_sort |
antigenic and genetic characterization of a divergent african virus, ikoma lyssavirus |
| description |
In 2009, a novel lyssavirus (subsequently named Ikoma lyssavirus, IKOV) was detected in the brain of an African civet (Civettictis civetta) with clinical rabies in the Serengeti National Park of Tanzania. The degree of nucleotide divergence between the genome of IKOV and those of other lyssaviruses predicted antigenic distinction from, and lack of protection provided by, available rabies vaccines. In addition, the index case was considered likely to be an incidental spillover event, and therefore the true reservoir of IKOV remained to be identified. The advent of sensitive molecular techniques has led to a rapid increase in the discovery of novel viruses. Detecting viral sequence alone, however, only allows for prediction of phenotypic characteristics and not their measurement. In the present study we describe the in vitro and in vivo characterization of IKOV, demonstrating that it is (1) pathogenic by peripheral inoculation in an animal model, (2) antigenically distinct from current rabies vaccine strains and (3) poorly neutralized by sera from humans and animals immunized against rabies. In a laboratory mouse model, no protection was elicited by a licensed rabies vaccine. We also investigated the role of bats as reservoirs of IKOV. We found no evidence for infection among 483 individuals of at least 13 bat species sampled across sites in the Serengeti and Southern Kenya. |
| publisher |
Society for General Microbiology |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983756/ |
| _version_ |
1612077116391686144 |