Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean

Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic...

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Main Authors:	Pedneault, Estelle, Galand, Pierre E., Potvin, Marianne, Tremblay, Jean-Éric, Lovejoy, Connie
Format:	Online
Language:	English
Published:	Nature Publishing Group 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983602/

id	pubmed-3983602
recordtype	oai_dc
spelling	pubmed-39836022014-04-11 Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean Pedneault, Estelle Galand, Pierre E. Potvin, Marianne Tremblay, Jean-Éric Lovejoy, Connie Article Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic samples from winter, spring and summer. AmoA genes, ureC genes and amoA transcripts were always present, but ureC transcripts were rarely detected. Over a 48 h light manipulation experiment amoA transcripts persisted under light and dark conditions, but not ureC transcripts. In addition, maxima for amoA transcript were nearer the surface compared to amoA genes. Clone libraries using DNA template recovered shallow and deep amoA clades but only the shallow clade was recovered from cDNA (from RNA). These results imply environmental control of amoA expression with direct or indirect light effects, and rare ureC expression despite its widespread occurrence in the Arctic Ocean. Nature Publishing Group 2014-04-11 /pmc/articles/PMC3983602/ /pubmed/24722490 http://dx.doi.org/10.1038/srep04661 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Pedneault, Estelle Galand, Pierre E. Potvin, Marianne Tremblay, Jean-Éric Lovejoy, Connie
spellingShingle	Pedneault, Estelle Galand, Pierre E. Potvin, Marianne Tremblay, Jean-Éric Lovejoy, Connie Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean
author_facet	Pedneault, Estelle Galand, Pierre E. Potvin, Marianne Tremblay, Jean-Éric Lovejoy, Connie
author_sort	Pedneault, Estelle
title	Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean
title_short	Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean
title_full	Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean
title_fullStr	Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean
title_full_unstemmed	Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean
title_sort	archaeal amoa and urec genes and their transcriptional activity in the arctic ocean
description	Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic samples from winter, spring and summer. AmoA genes, ureC genes and amoA transcripts were always present, but ureC transcripts were rarely detected. Over a 48 h light manipulation experiment amoA transcripts persisted under light and dark conditions, but not ureC transcripts. In addition, maxima for amoA transcript were nearer the surface compared to amoA genes. Clone libraries using DNA template recovered shallow and deep amoA clades but only the shallow clade was recovered from cDNA (from RNA). These results imply environmental control of amoA expression with direct or indirect light effects, and rare ureC expression despite its widespread occurrence in the Arctic Ocean.
publisher	Nature Publishing Group
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983602/
_version_	1612077081756172288

Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean

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