Carbon ion irradiation of the human prostate cancer cell line PC3: A whole genome microarray study

Carbon ion irradiation of the human prostate cancer cell line PC3: A whole genome microarray study

Hadrontherapy is a form of external radiation therapy, which uses beams of charged particles such as carbon ions. Compared to conventional radiotherapy with photons, the main advantage of carbon ion therapy is the precise dose localization along with an increased biological effectiveness. The first...

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Main Authors: SUETENS, ANNELIES, MOREELS, MARJAN, QUINTENS, ROEL, CHIRIOTTI, SABINA, TABURY, KEVIN, MICHAUX, ARLETTE, GRÉGOIRE, VINCENT, BAATOUT, SARAH
Format: Online
Language:English
Published: D.A. Spandidos 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977812/
id pubmed-3977812
recordtype oai_dc
spelling pubmed-39778122014-04-08 Carbon ion irradiation of the human prostate cancer cell line PC3: A whole genome microarray study SUETENS, ANNELIES MOREELS, MARJAN QUINTENS, ROEL CHIRIOTTI, SABINA TABURY, KEVIN MICHAUX, ARLETTE GRÉGOIRE, VINCENT BAATOUT, SARAH Articles Hadrontherapy is a form of external radiation therapy, which uses beams of charged particles such as carbon ions. Compared to conventional radiotherapy with photons, the main advantage of carbon ion therapy is the precise dose localization along with an increased biological effectiveness. The first results obtained from prostate cancer patients treated with carbon ion therapy showed good local tumor control and survival rates. In view of this advanced treatment modality we investigated the effects of irradiation with different beam qualities on gene expression changes in the PC3 prostate adenocarcinoma cell line. For this purpose, PC3 cells were irradiated with various doses (0.0, 0.5 and 2.0 Gy) of carbon ions (LET=33.7 keV/μm) at the beam of the Grand Accélérateur National d’Ions Lourds (Caen, France). Comparative experiments with X-rays were performed at the Belgian Nuclear Research Centre. Genome-wide gene expression was analyzed using microarrays. Our results show a downregulation in many genes involved in cell cycle and cell organization processes after 2.0 Gy irradiation. This effect was more pronounced after carbon ion irradiation compared with X-rays. Furthermore, we found a significant downregulation of many genes related to cell motility. Several of these changes were confirmed using qPCR. In addition, recurrence-free survival analysis of prostate cancer patients based on one of these motility genes (FN1) revealed that patients with low expression levels had a prolonged recurrence-free survival time, indicating that this gene may be a potential prognostic biomarker for prostate cancer. Understanding how different radiation qualities affect the cellular behavior of prostate cancer cells is important to improve the clinical outcome of cancer radiation therapy. D.A. Spandidos 2014-02-03 /pmc/articles/PMC3977812/ /pubmed/24504141 http://dx.doi.org/10.3892/ijo.2014.2287 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author SUETENS, ANNELIES
MOREELS, MARJAN
QUINTENS, ROEL
CHIRIOTTI, SABINA
TABURY, KEVIN
MICHAUX, ARLETTE
GRÉGOIRE, VINCENT
BAATOUT, SARAH
spellingShingle SUETENS, ANNELIES
MOREELS, MARJAN
QUINTENS, ROEL
CHIRIOTTI, SABINA
TABURY, KEVIN
MICHAUX, ARLETTE
GRÉGOIRE, VINCENT
BAATOUT, SARAH
Carbon ion irradiation of the human prostate cancer cell line PC3: A whole genome microarray study
author_facet SUETENS, ANNELIES
MOREELS, MARJAN
QUINTENS, ROEL
CHIRIOTTI, SABINA
TABURY, KEVIN
MICHAUX, ARLETTE
GRÉGOIRE, VINCENT
BAATOUT, SARAH
author_sort SUETENS, ANNELIES
title Carbon ion irradiation of the human prostate cancer cell line PC3: A whole genome microarray study
title_short Carbon ion irradiation of the human prostate cancer cell line PC3: A whole genome microarray study
title_full Carbon ion irradiation of the human prostate cancer cell line PC3: A whole genome microarray study
title_fullStr Carbon ion irradiation of the human prostate cancer cell line PC3: A whole genome microarray study
title_full_unstemmed Carbon ion irradiation of the human prostate cancer cell line PC3: A whole genome microarray study
title_sort carbon ion irradiation of the human prostate cancer cell line pc3: a whole genome microarray study
description Hadrontherapy is a form of external radiation therapy, which uses beams of charged particles such as carbon ions. Compared to conventional radiotherapy with photons, the main advantage of carbon ion therapy is the precise dose localization along with an increased biological effectiveness. The first results obtained from prostate cancer patients treated with carbon ion therapy showed good local tumor control and survival rates. In view of this advanced treatment modality we investigated the effects of irradiation with different beam qualities on gene expression changes in the PC3 prostate adenocarcinoma cell line. For this purpose, PC3 cells were irradiated with various doses (0.0, 0.5 and 2.0 Gy) of carbon ions (LET=33.7 keV/μm) at the beam of the Grand Accélérateur National d’Ions Lourds (Caen, France). Comparative experiments with X-rays were performed at the Belgian Nuclear Research Centre. Genome-wide gene expression was analyzed using microarrays. Our results show a downregulation in many genes involved in cell cycle and cell organization processes after 2.0 Gy irradiation. This effect was more pronounced after carbon ion irradiation compared with X-rays. Furthermore, we found a significant downregulation of many genes related to cell motility. Several of these changes were confirmed using qPCR. In addition, recurrence-free survival analysis of prostate cancer patients based on one of these motility genes (FN1) revealed that patients with low expression levels had a prolonged recurrence-free survival time, indicating that this gene may be a potential prognostic biomarker for prostate cancer. Understanding how different radiation qualities affect the cellular behavior of prostate cancer cells is important to improve the clinical outcome of cancer radiation therapy.
publisher D.A. Spandidos
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977812/
_version_ 1612074885179244544

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