Biogeographical Variation and Population Genetic Structure of Sporisorium scitamineum in Mainland China: Insights from ISSR and SP-SRAP Markers

A total of 100 Sporisorium scitamineum isolates were investigated by inter simple sequence repeat (ISSR) and single primer-sequence related amplified polymorphism (SP-SRAP) markers. These isolates were clearly assorted into three distinct clusters regardless of method used: either cluster analysis o...

Full description

Bibliographic Details
Main Authors:	Xu, Liping, Lu, Yunhai, You, Qian, Liu, Xiaolan, Grisham, Michael Paul, Pan, Yongbao, Que, Youxiong
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977103/

id	pubmed-3977103
recordtype	oai_dc
spelling	pubmed-39771032014-04-27 Biogeographical Variation and Population Genetic Structure of Sporisorium scitamineum in Mainland China: Insights from ISSR and SP-SRAP Markers Xu, Liping Lu, Yunhai You, Qian Liu, Xiaolan Grisham, Michael Paul Pan, Yongbao Que, Youxiong Research Article A total of 100 Sporisorium scitamineum isolates were investigated by inter simple sequence repeat (ISSR) and single primer-sequence related amplified polymorphism (SP-SRAP) markers. These isolates were clearly assorted into three distinct clusters regardless of method used: either cluster analysis or by principal component analysis (PCA) of the ISSR, SP-SRAP, or ISSR + SP-SRAP data set. The total gene diversity (H t) and gene diversity between subpopulations (H s) were estimated to be 0.34 to 0.38 and 0.22 to 0.29, respectively, by analyzing separately the ISSR and SP-SRAP data sets, and to be 0.26–0.36 by analyzing ISSR + SP-SRAP data set. The gene diversity attributable to differentiation among populations (G st) was estimated to be 0.35 and 0.22, and the gene flow (Nm) was 0.94 and 1.78, respectively, when analyzing separately ISSR and SP-SRAP data set, and was 0.27 and 1.33, respectively, when analyzing ISSR + SP-SRAP data set. Our study showed that there is considerable genetic variation in the analyzed 100 isolates, and the environmental heterogeneity has played an important role for this observed high degree of variation. The genetic differentiation of sugarcane smut fungus depends to a large extent on the heterogeneity of their habitats and is the result of long-term adaptations of pathogens to their ecological environments. Hindawi Publishing Corporation 2014-03-17 /pmc/articles/PMC3977103/ /pubmed/24772015 http://dx.doi.org/10.1155/2014/296020 Text en Copyright © 2014 Liping Xu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Xu, Liping Lu, Yunhai You, Qian Liu, Xiaolan Grisham, Michael Paul Pan, Yongbao Que, Youxiong
spellingShingle	Xu, Liping Lu, Yunhai You, Qian Liu, Xiaolan Grisham, Michael Paul Pan, Yongbao Que, Youxiong Biogeographical Variation and Population Genetic Structure of Sporisorium scitamineum in Mainland China: Insights from ISSR and SP-SRAP Markers
author_facet	Xu, Liping Lu, Yunhai You, Qian Liu, Xiaolan Grisham, Michael Paul Pan, Yongbao Que, Youxiong
author_sort	Xu, Liping
title	Biogeographical Variation and Population Genetic Structure of Sporisorium scitamineum in Mainland China: Insights from ISSR and SP-SRAP Markers
title_short	Biogeographical Variation and Population Genetic Structure of Sporisorium scitamineum in Mainland China: Insights from ISSR and SP-SRAP Markers
title_full	Biogeographical Variation and Population Genetic Structure of Sporisorium scitamineum in Mainland China: Insights from ISSR and SP-SRAP Markers
title_fullStr	Biogeographical Variation and Population Genetic Structure of Sporisorium scitamineum in Mainland China: Insights from ISSR and SP-SRAP Markers
title_full_unstemmed	Biogeographical Variation and Population Genetic Structure of Sporisorium scitamineum in Mainland China: Insights from ISSR and SP-SRAP Markers
title_sort	biogeographical variation and population genetic structure of sporisorium scitamineum in mainland china: insights from issr and sp-srap markers
description	A total of 100 Sporisorium scitamineum isolates were investigated by inter simple sequence repeat (ISSR) and single primer-sequence related amplified polymorphism (SP-SRAP) markers. These isolates were clearly assorted into three distinct clusters regardless of method used: either cluster analysis or by principal component analysis (PCA) of the ISSR, SP-SRAP, or ISSR + SP-SRAP data set. The total gene diversity (H t) and gene diversity between subpopulations (H s) were estimated to be 0.34 to 0.38 and 0.22 to 0.29, respectively, by analyzing separately the ISSR and SP-SRAP data sets, and to be 0.26–0.36 by analyzing ISSR + SP-SRAP data set. The gene diversity attributable to differentiation among populations (G st) was estimated to be 0.35 and 0.22, and the gene flow (Nm) was 0.94 and 1.78, respectively, when analyzing separately ISSR and SP-SRAP data set, and was 0.27 and 1.33, respectively, when analyzing ISSR + SP-SRAP data set. Our study showed that there is considerable genetic variation in the analyzed 100 isolates, and the environmental heterogeneity has played an important role for this observed high degree of variation. The genetic differentiation of sugarcane smut fungus depends to a large extent on the heterogeneity of their habitats and is the result of long-term adaptations of pathogens to their ecological environments.
publisher	Hindawi Publishing Corporation
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977103/
_version_	1612074681348653056

Biogeographical Variation and Population Genetic Structure of Sporisorium scitamineum in Mainland China: Insights from ISSR and SP-SRAP Markers

Similar Items