Action and Signaling of Lysophosphatidylethanolamine in MDA-MB-231 Breast Cancer Cells

Action and Signaling of Lysophosphatidylethanolamine in MDA-MB-231 Breast Cancer Cells

Previously, we reported that lysophosphatidylethanolamine (LPE), a lyso-type metabolite of phosphatidylethanolamine, can increase intracellular Ca2+ ([Ca2+]i) via type 1 lysophosphatidic acid (LPA) receptor (LPA1) and CD97, an adhesion G-protein-coupled receptor (GPCR), in MDA-MB-231 breast cancer c...

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Main Authors: Park, Soo-Jin, Lee, Kyoung-Pil, Im, Dong-Soon
Format: Online
Language:English
Published: The Korean Society of Applied Pharmacology 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975480/
id pubmed-3975480
recordtype oai_dc
spelling pubmed-39754802014-04-21 Action and Signaling of Lysophosphatidylethanolamine in MDA-MB-231 Breast Cancer Cells Park, Soo-Jin Lee, Kyoung-Pil Im, Dong-Soon Original Article Previously, we reported that lysophosphatidylethanolamine (LPE), a lyso-type metabolite of phosphatidylethanolamine, can increase intracellular Ca2+ ([Ca2+]i) via type 1 lysophosphatidic acid (LPA) receptor (LPA1) and CD97, an adhesion G-protein-coupled receptor (GPCR), in MDA-MB-231 breast cancer cells. Furthermore, LPE signaling was suggested as like LPA1/CD97-Gi/o proteins-phospholipase C-IP3-Ca2+ increase in these cells. In the present study, we further investigated actions of LPE not only in the [Ca2+]i increasing effect but also in cell proliferation and migration in MDA-MB-231 breast cancer cells. We utilized chemically different LPEs and a specific inhibitor of LPA1, AM-095 in comparison with responses in SK-OV3 ovarian cancer cells. It was found that LPE-induced Ca2+ response in MDA-MB-231 cells was evoked in a different manner to that in SK-OV3 cells in terms of structural requirements. AM-095 inhibited LPE-induced Ca2+ response and cell proliferation in MDA-MB-231 cells, but not in SK-OV3 cells, supporting LPA1 involvement only in MDA-MB-231 cells. LPA had significant effects on cell proliferation and migration in MDA-MB-231 cells, whereas LPE had less or no significant effect. However, LPE modulations of MAPKs (ERK1/2, JNK and p38 MAPK) was not different to those by LPA in the cells. These data support the involvement of LPA1 in LPE-induced Ca2+ response and cell proliferation in breast MDA-MB-231 cells but unknown GPCRs (not LPA1) in LPE-induced responses in SK-OV3 cells. Furthermore, although LPE and LPA utilized LPA1, LPA utilized more signaling cascades than LPE, resulting in stronger responses by LPA in proliferation and migration than LPE in MDA-MB-231 cells. The Korean Society of Applied Pharmacology 2014-03 /pmc/articles/PMC3975480/ /pubmed/24753818 http://dx.doi.org/10.4062/biomolther.2013.110 Text en Copyright © 2014 The Korean Society of Applied Pharmacology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Park, Soo-Jin
Lee, Kyoung-Pil
Im, Dong-Soon
spellingShingle Park, Soo-Jin
Lee, Kyoung-Pil
Im, Dong-Soon
Action and Signaling of Lysophosphatidylethanolamine in MDA-MB-231 Breast Cancer Cells
author_facet Park, Soo-Jin
Lee, Kyoung-Pil
Im, Dong-Soon
author_sort Park, Soo-Jin
title Action and Signaling of Lysophosphatidylethanolamine in MDA-MB-231 Breast Cancer Cells
title_short Action and Signaling of Lysophosphatidylethanolamine in MDA-MB-231 Breast Cancer Cells
title_full Action and Signaling of Lysophosphatidylethanolamine in MDA-MB-231 Breast Cancer Cells
title_fullStr Action and Signaling of Lysophosphatidylethanolamine in MDA-MB-231 Breast Cancer Cells
title_full_unstemmed Action and Signaling of Lysophosphatidylethanolamine in MDA-MB-231 Breast Cancer Cells
title_sort action and signaling of lysophosphatidylethanolamine in mda-mb-231 breast cancer cells
description Previously, we reported that lysophosphatidylethanolamine (LPE), a lyso-type metabolite of phosphatidylethanolamine, can increase intracellular Ca2+ ([Ca2+]i) via type 1 lysophosphatidic acid (LPA) receptor (LPA1) and CD97, an adhesion G-protein-coupled receptor (GPCR), in MDA-MB-231 breast cancer cells. Furthermore, LPE signaling was suggested as like LPA1/CD97-Gi/o proteins-phospholipase C-IP3-Ca2+ increase in these cells. In the present study, we further investigated actions of LPE not only in the [Ca2+]i increasing effect but also in cell proliferation and migration in MDA-MB-231 breast cancer cells. We utilized chemically different LPEs and a specific inhibitor of LPA1, AM-095 in comparison with responses in SK-OV3 ovarian cancer cells. It was found that LPE-induced Ca2+ response in MDA-MB-231 cells was evoked in a different manner to that in SK-OV3 cells in terms of structural requirements. AM-095 inhibited LPE-induced Ca2+ response and cell proliferation in MDA-MB-231 cells, but not in SK-OV3 cells, supporting LPA1 involvement only in MDA-MB-231 cells. LPA had significant effects on cell proliferation and migration in MDA-MB-231 cells, whereas LPE had less or no significant effect. However, LPE modulations of MAPKs (ERK1/2, JNK and p38 MAPK) was not different to those by LPA in the cells. These data support the involvement of LPA1 in LPE-induced Ca2+ response and cell proliferation in breast MDA-MB-231 cells but unknown GPCRs (not LPA1) in LPE-induced responses in SK-OV3 cells. Furthermore, although LPE and LPA utilized LPA1, LPA utilized more signaling cascades than LPE, resulting in stronger responses by LPA in proliferation and migration than LPE in MDA-MB-231 cells.
publisher The Korean Society of Applied Pharmacology
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975480/
_version_ 1612074148397318144

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