Template-directed Chemical Ligation to Obtain 3′-3′ and 5′-5′ Phosphodiester DNA Linkages

Template-directed Chemical Ligation to Obtain 3′-3′ and 5′-5′ Phosphodiester DNA Linkages

Up to now, the direct ligation of two DNA fragments with opposite directions to obtain 3′-3′ or 5′-5′ phosphate ester bonds is still challenging. The only way to obtain DNA oligonucleotides containing a 3′-3′ or 5′-5′ inversion of polarity sites is based on professional DNA chemical synthesis. Herei...

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Bibliographic Details
Main Authors: Chen, Haodong, Du, Feng, Chen, Gangyi, Streckenbach, Frank, Yasmeen, Afshan, Zhao, Yun, Tang, Zhuo
Format: Online
Language:English
Published: Nature Publishing Group 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975322/

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975322/

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