Bone marrow stem/progenitor cell mobilization in C57BL/6J and BALB/c mice

Bone marrow (BM) has been considered as a reservoir of stem/progenitor cells which are able to differentiate into ectodermal, endodermal, and mesodermal origins in vitro as well as in vivo. Following adequate stimulation, such as granulocyte stimulating factor (G-CSF) or AMD3100, BM resident stem/pr...

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Main Authors:	Lee, Hakmo, Che, Jeong-Hwan, Oh, Ju Eun, Chung, Sung Soo, Jung, Hye Seung, Park, Kyong Soo
Format:	Online
Language:	English
Published:	Korean Association for Laboratory Animal Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973806/

id	pubmed-3973806
recordtype	oai_dc
spelling	pubmed-39738062014-04-04 Bone marrow stem/progenitor cell mobilization in C57BL/6J and BALB/c mice Lee, Hakmo Che, Jeong-Hwan Oh, Ju Eun Chung, Sung Soo Jung, Hye Seung Park, Kyong Soo Original Article Bone marrow (BM) has been considered as a reservoir of stem/progenitor cells which are able to differentiate into ectodermal, endodermal, and mesodermal origins in vitro as well as in vivo. Following adequate stimulation, such as granulocyte stimulating factor (G-CSF) or AMD3100, BM resident stem/progenitor cells (BMSPCs) can be mobilized to peripheral blood. Several host-related factors are known to participate in this mobilization process. In fact, a significant number of donors are resistant to G-CSF induced mobilization protocols. AMD3100 is currently used in combination with G-CSF. However, information regarding host-related factors which may influence the AMD3100 directed mobilization is extremely limited. In this study, we were to get some more knowledge on the host-related factors that affect the efficiency of AMD3100 induced mobilization by employing in vivo mobilization experiments. As a result, we found that C57BL/6J mice are more sensitive to AMD3100 but less sensitive to G-CSF which promotes the proliferation of BMSPCs. We excluded S1P as one of the host related factor which influences AMD3100 directed mobilization because pre-treatment of S1P receptor antagonist FTY720 did not inhibit BMSPC mobilization. Further in vitro experiments revealed that BALB/c mice, compared to C57BL/6J mice, have less BMSPCs which migrate in response to host related factors such as sphingosine-1-phosphate (S1P) and to CXCL12. We conclude that AMD3100-directed mobilization depends on the number of BMSPCs rather than on the host-related factors. These results suggest that the combination of AMD3100 and G-CSF is co-operative and is optimal for the mobilization of BMSPCs. Korean Association for Laboratory Animal Science 2014-03 2014-03-24 /pmc/articles/PMC3973806/ /pubmed/24707300 http://dx.doi.org/10.5625/lar.2014.30.1.14 Text en Copyright © 2014 Korean Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lee, Hakmo Che, Jeong-Hwan Oh, Ju Eun Chung, Sung Soo Jung, Hye Seung Park, Kyong Soo
spellingShingle	Lee, Hakmo Che, Jeong-Hwan Oh, Ju Eun Chung, Sung Soo Jung, Hye Seung Park, Kyong Soo Bone marrow stem/progenitor cell mobilization in C57BL/6J and BALB/c mice
author_facet	Lee, Hakmo Che, Jeong-Hwan Oh, Ju Eun Chung, Sung Soo Jung, Hye Seung Park, Kyong Soo
author_sort	Lee, Hakmo
title	Bone marrow stem/progenitor cell mobilization in C57BL/6J and BALB/c mice
title_short	Bone marrow stem/progenitor cell mobilization in C57BL/6J and BALB/c mice
title_full	Bone marrow stem/progenitor cell mobilization in C57BL/6J and BALB/c mice
title_fullStr	Bone marrow stem/progenitor cell mobilization in C57BL/6J and BALB/c mice
title_full_unstemmed	Bone marrow stem/progenitor cell mobilization in C57BL/6J and BALB/c mice
title_sort	bone marrow stem/progenitor cell mobilization in c57bl/6j and balb/c mice
description	Bone marrow (BM) has been considered as a reservoir of stem/progenitor cells which are able to differentiate into ectodermal, endodermal, and mesodermal origins in vitro as well as in vivo. Following adequate stimulation, such as granulocyte stimulating factor (G-CSF) or AMD3100, BM resident stem/progenitor cells (BMSPCs) can be mobilized to peripheral blood. Several host-related factors are known to participate in this mobilization process. In fact, a significant number of donors are resistant to G-CSF induced mobilization protocols. AMD3100 is currently used in combination with G-CSF. However, information regarding host-related factors which may influence the AMD3100 directed mobilization is extremely limited. In this study, we were to get some more knowledge on the host-related factors that affect the efficiency of AMD3100 induced mobilization by employing in vivo mobilization experiments. As a result, we found that C57BL/6J mice are more sensitive to AMD3100 but less sensitive to G-CSF which promotes the proliferation of BMSPCs. We excluded S1P as one of the host related factor which influences AMD3100 directed mobilization because pre-treatment of S1P receptor antagonist FTY720 did not inhibit BMSPC mobilization. Further in vitro experiments revealed that BALB/c mice, compared to C57BL/6J mice, have less BMSPCs which migrate in response to host related factors such as sphingosine-1-phosphate (S1P) and to CXCL12. We conclude that AMD3100-directed mobilization depends on the number of BMSPCs rather than on the host-related factors. These results suggest that the combination of AMD3100 and G-CSF is co-operative and is optimal for the mobilization of BMSPCs.
publisher	Korean Association for Laboratory Animal Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973806/
_version_	1612073511337066496

Bone marrow stem/progenitor cell mobilization in C57BL/6J and BALB/c mice

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