AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation

In the present study, we investigated the 3′ untranslated region (UTR) of the mouse core clock gene cryptochrome 1 (Cry1) at the post-transcriptional level, particularly its translational regulation. Interestingly, the 3′UTR of Cry1 mRNA decreased its mRNA levels but increased protein amounts. The 3...

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Main Authors:	Lee, Kyung-Ha, Kim, Sung-Hoon, Kim, Hyo-Jin, Kim, Wanil, Lee, Hwa-Rim, Jung, Youngseob, Choi, Jung-Hyun, Hong, Ka Young, Jang, Sung Key, Kim, Kyong-Tai
Format:	Online
Language:	English
Published:	Oxford University Press 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973335/

id	pubmed-3973335
recordtype	oai_dc
spelling	pubmed-39733352014-04-04 AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation Lee, Kyung-Ha Kim, Sung-Hoon Kim, Hyo-Jin Kim, Wanil Lee, Hwa-Rim Jung, Youngseob Choi, Jung-Hyun Hong, Ka Young Jang, Sung Key Kim, Kyong-Tai Gene Regulation, Chromatin and Epigenetics In the present study, we investigated the 3′ untranslated region (UTR) of the mouse core clock gene cryptochrome 1 (Cry1) at the post-transcriptional level, particularly its translational regulation. Interestingly, the 3′UTR of Cry1 mRNA decreased its mRNA levels but increased protein amounts. The 3′UTR is widely known to function as a cis-acting element of mRNA degradation. The 3′UTR also provides a binding site for microRNA and mainly suppresses translation of target mRNAs. We found that AU-rich element RNA binding protein 1 (AUF1) directly binds to the Cry1 3′UTR and regulates translation of Cry1 mRNA. AUF1 interacted with eukaryotic translation initiation factor 3 subunit B and also directly associated with ribosomal protein S3 or ribosomal protein S14, resulting in translation of Cry1 mRNA in a 3′UTR-dependent manner. Expression of cytoplasmic AUF1 and binding of AUF1 to the Cry1 3′UTR were parallel to the circadian CRY1 protein profile. Our results suggest that the 3′UTR of Cry1 is important for its rhythmic translation, and AUF1 bound to the 3′UTR facilitates interaction with the 5′ end of mRNA by interacting with translation initiation factors and recruiting the 40S ribosomal subunit to initiate translation of Cry1 mRNA. Oxford University Press 2014-04 2014-01-13 /pmc/articles/PMC3973335/ /pubmed/24423872 http://dx.doi.org/10.1093/nar/gkt1379 Text en © The Author(s) 2014. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lee, Kyung-Ha Kim, Sung-Hoon Kim, Hyo-Jin Kim, Wanil Lee, Hwa-Rim Jung, Youngseob Choi, Jung-Hyun Hong, Ka Young Jang, Sung Key Kim, Kyong-Tai
spellingShingle	Lee, Kyung-Ha Kim, Sung-Hoon Kim, Hyo-Jin Kim, Wanil Lee, Hwa-Rim Jung, Youngseob Choi, Jung-Hyun Hong, Ka Young Jang, Sung Key Kim, Kyong-Tai AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation
author_facet	Lee, Kyung-Ha Kim, Sung-Hoon Kim, Hyo-Jin Kim, Wanil Lee, Hwa-Rim Jung, Youngseob Choi, Jung-Hyun Hong, Ka Young Jang, Sung Key Kim, Kyong-Tai
author_sort	Lee, Kyung-Ha
title	AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation
title_short	AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation
title_full	AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation
title_fullStr	AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation
title_full_unstemmed	AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation
title_sort	auf1 contributes to cryptochrome1 mrna degradation and rhythmic translation
description	In the present study, we investigated the 3′ untranslated region (UTR) of the mouse core clock gene cryptochrome 1 (Cry1) at the post-transcriptional level, particularly its translational regulation. Interestingly, the 3′UTR of Cry1 mRNA decreased its mRNA levels but increased protein amounts. The 3′UTR is widely known to function as a cis-acting element of mRNA degradation. The 3′UTR also provides a binding site for microRNA and mainly suppresses translation of target mRNAs. We found that AU-rich element RNA binding protein 1 (AUF1) directly binds to the Cry1 3′UTR and regulates translation of Cry1 mRNA. AUF1 interacted with eukaryotic translation initiation factor 3 subunit B and also directly associated with ribosomal protein S3 or ribosomal protein S14, resulting in translation of Cry1 mRNA in a 3′UTR-dependent manner. Expression of cytoplasmic AUF1 and binding of AUF1 to the Cry1 3′UTR were parallel to the circadian CRY1 protein profile. Our results suggest that the 3′UTR of Cry1 is important for its rhythmic translation, and AUF1 bound to the 3′UTR facilitates interaction with the 5′ end of mRNA by interacting with translation initiation factors and recruiting the 40S ribosomal subunit to initiate translation of Cry1 mRNA.
publisher	Oxford University Press
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973335/
_version_	1612073363389284352

AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation

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