Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin

Prior studies identified T cells, B cells, and macrophages in the inflammatory infiltrate and up-regulation of their protein products in discoid lupus erythematosus (DLE) skin; however, they lacked rigorous analyses to define their specific locations in skin. Thus, we compared expressions of selecte...

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Main Authors:	Thorpe, Ryan B., Gray, Anna, Kumar, Kirthi R., Susa, Joseph S., Chong, Benjamin F.
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972874/

id	pubmed-3972874
recordtype	oai_dc
spelling	pubmed-39728742014-04-17 Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin Thorpe, Ryan B. Gray, Anna Kumar, Kirthi R. Susa, Joseph S. Chong, Benjamin F. Research Article Prior studies identified T cells, B cells, and macrophages in the inflammatory infiltrate and up-regulation of their protein products in discoid lupus erythematosus (DLE) skin; however, they lacked rigorous analyses to define their specific locations in skin. Thus, we compared expressions of selected T cell, B cell, and macrophage markers in five areas of DLE, psoriasis, and normal skin. Immunostainings for CD3, CD4, CD8, CD20, CD68, CXCR3, CXCL10, and TIA-1 were performed in biopsies of 23 DLE lesional skin, 11 psoriasis lesional skin, and 5 normal skin. Three independent observers used a graded scale to rate each marker's presence in the epidermis, dermatoepidermal junction (DEJ), perivascular area, periadnexal area, and deep dermis. DLE lesional skin contained an increased abundance of CD3+, CD8+, and CD68+ cells at the DEJ, and CD20+ and CD68+ cells in the periadnexal area versus psoriasis and normal skin. CXCR3, CXCL10, and TIA-1 were elevated in periadnexal sites of DLE lesional skin versus psoriasis lesional skin. The aggregation of T cells, B cells, macrophages, and their protein products (CXCR3, CXCL10, and TIA-1) in the DEJ and periadnexal area of DLE lesional skin may contribute to the pathology of DLE through a coordinated, sophisticated process. Hindawi Publishing Corporation 2014-03-11 /pmc/articles/PMC3972874/ /pubmed/24744689 http://dx.doi.org/10.1155/2014/925805 Text en Copyright © 2014 Ryan B. Thorpe et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Thorpe, Ryan B. Gray, Anna Kumar, Kirthi R. Susa, Joseph S. Chong, Benjamin F.
spellingShingle	Thorpe, Ryan B. Gray, Anna Kumar, Kirthi R. Susa, Joseph S. Chong, Benjamin F. Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin
author_facet	Thorpe, Ryan B. Gray, Anna Kumar, Kirthi R. Susa, Joseph S. Chong, Benjamin F.
author_sort	Thorpe, Ryan B.
title	Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin
title_short	Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin
title_full	Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin
title_fullStr	Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin
title_full_unstemmed	Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin
title_sort	site-specific analysis of inflammatory markers in discoid lupus erythematosus skin
description	Prior studies identified T cells, B cells, and macrophages in the inflammatory infiltrate and up-regulation of their protein products in discoid lupus erythematosus (DLE) skin; however, they lacked rigorous analyses to define their specific locations in skin. Thus, we compared expressions of selected T cell, B cell, and macrophage markers in five areas of DLE, psoriasis, and normal skin. Immunostainings for CD3, CD4, CD8, CD20, CD68, CXCR3, CXCL10, and TIA-1 were performed in biopsies of 23 DLE lesional skin, 11 psoriasis lesional skin, and 5 normal skin. Three independent observers used a graded scale to rate each marker's presence in the epidermis, dermatoepidermal junction (DEJ), perivascular area, periadnexal area, and deep dermis. DLE lesional skin contained an increased abundance of CD3+, CD8+, and CD68+ cells at the DEJ, and CD20+ and CD68+ cells in the periadnexal area versus psoriasis and normal skin. CXCR3, CXCL10, and TIA-1 were elevated in periadnexal sites of DLE lesional skin versus psoriasis lesional skin. The aggregation of T cells, B cells, macrophages, and their protein products (CXCR3, CXCL10, and TIA-1) in the DEJ and periadnexal area of DLE lesional skin may contribute to the pathology of DLE through a coordinated, sophisticated process.
publisher	Hindawi Publishing Corporation
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972874/
_version_	1612073219390439424

Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin

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