DsRed2 transient expression in Culex quinquefasciatus mosquitoes
Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemoly...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970632/ |
| Summary: | Culex quinquefasciatus mosquitoes have been successfully
genetically modified only once, despite the efforts of several laboratories to
transform and establish a stable strain. We have developed a transient gene
expression method, in Culex, that delivers plasmid DNA directly
to the mosquito haemolymph and additional tissues. We were able to express
DsRed2 fluorescent protein in adult Cx.
quinquefasciatus mosquitoes by injecting plasmids directly into
their thorax. The expression of DsRed2 in adult Cx.
quinquefasciatus mosquitoes is an important stepping stone to
genetic transformation and the potential use of new control strategies and
genetic interactions. |
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