Bacterial expression of human kynurenine 3-monooxygenase: Solubility, activity, purification☆
•This is the first report of soluble and active bacterially expressed human KMO protein.•Partial purification of the enzyme was achieved and the two protein co-elutants identified.•Steady state kinetic parameters were comparable to those reported for mammalian expressed.•The C-terminal membrane targ...
| Main Authors: | , , , , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Academic Press
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969302/ |