Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions☆
Orsay virus is the first identified virus that is capable of naturally infecting Caenorhabditis elegans. Although it is most closely related to nodaviruses, Orsay virus differs from nodaviruses in its genome organization. In particular, the Orsay virus RNA2 segment encodes a putative novel protein o...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Academic Press
2014
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969245/ |
| id |
pubmed-3969245 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-39692452014-03-31 Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions☆ Jiang, Hongbing Franz, Carl J. Wu, Guang Renshaw, Hilary Zhao, Guoyan Firth, Andrew E. Wang, David Article Orsay virus is the first identified virus that is capable of naturally infecting Caenorhabditis elegans. Although it is most closely related to nodaviruses, Orsay virus differs from nodaviruses in its genome organization. In particular, the Orsay virus RNA2 segment encodes a putative novel protein of unknown function, termed delta, which is absent from all known nodaviruses. Here we present evidence that Orsay virus utilizes a ribosomal frameshifting strategy to express a novel fusion protein from the viral capsid (alpha) and delta ORFs. Moreover, the fusion protein was detected in purified virus fractions, demonstrating that it is most likely incorporated into Orsay virions. Furthermore, N-terminal sequencing of both the fusion protein and the capsid protein demonstrated that these proteins must be translated from a non-canonical initiation site. While the function of the alpha–delta fusion remains cryptic, these studies provide novel insights into the fundamental properties of this new clade of viruses. Academic Press 2014-02 /pmc/articles/PMC3969245/ /pubmed/24503084 http://dx.doi.org/10.1016/j.virol.2013.12.016 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Jiang, Hongbing Franz, Carl J. Wu, Guang Renshaw, Hilary Zhao, Guoyan Firth, Andrew E. Wang, David |
| spellingShingle |
Jiang, Hongbing Franz, Carl J. Wu, Guang Renshaw, Hilary Zhao, Guoyan Firth, Andrew E. Wang, David Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions☆ |
| author_facet |
Jiang, Hongbing Franz, Carl J. Wu, Guang Renshaw, Hilary Zhao, Guoyan Firth, Andrew E. Wang, David |
| author_sort |
Jiang, Hongbing |
| title |
Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions☆ |
| title_short |
Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions☆ |
| title_full |
Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions☆ |
| title_fullStr |
Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions☆ |
| title_full_unstemmed |
Orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions☆ |
| title_sort |
orsay virus utilizes ribosomal frameshifting to express a novel protein that is incorporated into virions☆ |
| description |
Orsay virus is the first identified virus that is capable of naturally infecting Caenorhabditis elegans. Although it is most closely related to nodaviruses, Orsay virus differs from nodaviruses in its genome organization. In particular, the Orsay virus RNA2 segment encodes a putative novel protein of unknown function, termed delta, which is absent from all known nodaviruses. Here we present evidence that Orsay virus utilizes a ribosomal frameshifting strategy to express a novel fusion protein from the viral capsid (alpha) and delta ORFs. Moreover, the fusion protein was detected in purified virus fractions, demonstrating that it is most likely incorporated into Orsay virions. Furthermore, N-terminal sequencing of both the fusion protein and the capsid protein demonstrated that these proteins must be translated from a non-canonical initiation site. While the function of the alpha–delta fusion remains cryptic, these studies provide novel insights into the fundamental properties of this new clade of viruses. |
| publisher |
Academic Press |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969245/ |
| _version_ |
1612072266545233920 |