Detection of mutations in KLHL3 and CUL3 in families with FHHt (familial hyperkalaemic hypertension or Gordon's syndrome)
The study of families with rare inherited forms of hypo- and hyper-tension has been one of the most successful strategies to probe the molecular pathophysiology of blood pressure control and has revealed dysregulation of distal nephron Na+ reabsorption to be a common mechanism. FHHt (familial hyperk...
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| Format: | Online |
| Language: | English |
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Portland Press Ltd.
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963521/ |
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pubmed-39635212014-03-24 Detection of mutations in KLHL3 and CUL3 in families with FHHt (familial hyperkalaemic hypertension or Gordon's syndrome) Glover, Mark Ware, James S. Henry, Amanda Wolley, Martin Walsh, Roddy Wain, Louise V. Xu, Shengxin Van’t Hoff, William G. Tobin, Martin D. Hall, Ian P. Cook, Stuart Gordon, Richard D. Stowasser, Michael O’Shaughnessy, Kevin M. Original Paper The study of families with rare inherited forms of hypo- and hyper-tension has been one of the most successful strategies to probe the molecular pathophysiology of blood pressure control and has revealed dysregulation of distal nephron Na+ reabsorption to be a common mechanism. FHHt (familial hyperkalaemic hypertension; also known as Gordon's syndrome) is a salt-dependent form of hypertension caused by mutations in the regulators of the thiazide-sensitive Na+–Cl− co-transporter NCC [also known as SLC12A3 (solute carrier family 12 member 3)] and is effectively treated by thiazide diuretics and/or dietary salt restriction. Variation in at least four genes can cause FHHt, including WNK1 [With No lysine (=K) 1] and WNK4, KLHL3 (kelch-like family member 3), and CUL3 (cullin 3). In the present study we have identified novel disease-causing variants in CUL3 and KLHL3 segregating in 63% of the pedigrees with previously unexplained FHHt, confirming the importance of these recently described FHHt genes. We have demonstrated conclusively, in two unrelated affected individuals, that rare intronic variants in CUL3 cause the skipping of exon 9 as has been proposed previously. KLHL3 variants all occur in kelch-repeat domains and so probably disrupt WNK complex binding. We have found no evidence of any plausible disease-causing variants within SLC4A8 (an alternative thiazide-sensitive sodium transporter) in this population. The results of the present study support the existing evidence that the CUL3 and KLHL3 gene products are physiologically important regulators of thiazide-sensitive distal nephron NaCl reabsorption, and hence potentially interesting novel anti-hypertensive drug targets. As a third of our non-WNK FHHt families do not have plausible CUL3 or KLHL3 variants, there are probably additional, as yet undiscovered, regulators of the thiazide-sensitive pathways. Portland Press Ltd. 2014-02-03 2014-05-01 /pmc/articles/PMC3963521/ /pubmed/24266877 http://dx.doi.org/10.1042/CS20130326 Text en © 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Glover, Mark Ware, James S. Henry, Amanda Wolley, Martin Walsh, Roddy Wain, Louise V. Xu, Shengxin Van’t Hoff, William G. Tobin, Martin D. Hall, Ian P. Cook, Stuart Gordon, Richard D. Stowasser, Michael O’Shaughnessy, Kevin M. |
| spellingShingle |
Glover, Mark Ware, James S. Henry, Amanda Wolley, Martin Walsh, Roddy Wain, Louise V. Xu, Shengxin Van’t Hoff, William G. Tobin, Martin D. Hall, Ian P. Cook, Stuart Gordon, Richard D. Stowasser, Michael O’Shaughnessy, Kevin M. Detection of mutations in KLHL3 and CUL3 in families with FHHt (familial hyperkalaemic hypertension or Gordon's syndrome) |
| author_facet |
Glover, Mark Ware, James S. Henry, Amanda Wolley, Martin Walsh, Roddy Wain, Louise V. Xu, Shengxin Van’t Hoff, William G. Tobin, Martin D. Hall, Ian P. Cook, Stuart Gordon, Richard D. Stowasser, Michael O’Shaughnessy, Kevin M. |
| author_sort |
Glover, Mark |
| title |
Detection of mutations in KLHL3 and CUL3 in
families with FHHt (familial hyperkalaemic hypertension or Gordon's syndrome) |
| title_short |
Detection of mutations in KLHL3 and CUL3 in
families with FHHt (familial hyperkalaemic hypertension or Gordon's syndrome) |
| title_full |
Detection of mutations in KLHL3 and CUL3 in
families with FHHt (familial hyperkalaemic hypertension or Gordon's syndrome) |
| title_fullStr |
Detection of mutations in KLHL3 and CUL3 in
families with FHHt (familial hyperkalaemic hypertension or Gordon's syndrome) |
| title_full_unstemmed |
Detection of mutations in KLHL3 and CUL3 in
families with FHHt (familial hyperkalaemic hypertension or Gordon's syndrome) |
| title_sort |
detection of mutations in klhl3 and cul3 in
families with fhht (familial hyperkalaemic hypertension or gordon's syndrome) |
| description |
The study of families with rare inherited forms of hypo- and hyper-tension has been one of the
most successful strategies to probe the molecular pathophysiology of blood pressure control and has
revealed dysregulation of distal nephron Na+ reabsorption to be a common mechanism. FHHt
(familial hyperkalaemic hypertension; also known as Gordon's syndrome) is a salt-dependent
form of hypertension caused by mutations in the regulators of the thiazide-sensitive
Na+–Cl− co-transporter NCC [also known as SLC12A3 (solute
carrier family 12 member 3)] and is effectively treated by thiazide diuretics and/or dietary salt
restriction. Variation in at least four genes can cause FHHt, including WNK1 [With
No lysine (=K) 1] and WNK4, KLHL3 (kelch-like family member 3),
and CUL3 (cullin 3). In the present study we have identified novel disease-causing
variants in CUL3 and KLHL3 segregating in 63% of the pedigrees
with previously unexplained FHHt, confirming the importance of these recently described FHHt genes.
We have demonstrated conclusively, in two unrelated affected individuals, that rare intronic
variants in CUL3 cause the skipping of exon 9 as has been proposed previously.
KLHL3 variants all occur in kelch-repeat domains and so probably disrupt WNK
complex binding. We have found no evidence of any plausible disease-causing variants within
SLC4A8 (an alternative thiazide-sensitive sodium transporter) in this population.
The results of the present study support the existing evidence that the CUL3 and
KLHL3 gene products are physiologically important regulators of thiazide-sensitive
distal nephron NaCl reabsorption, and hence potentially interesting novel anti-hypertensive drug
targets. As a third of our non-WNK FHHt families do not have plausible
CUL3 or KLHL3 variants, there are probably additional, as yet
undiscovered, regulators of the thiazide-sensitive pathways. |
| publisher |
Portland Press Ltd. |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963521/ |
| _version_ |
1612070559783321600 |