Restriction Enzyme Body Doubles and PCR Cloning: On the General Use of Type IIS Restriction Enzymes for Cloning

Restriction Enzyme Body Doubles and PCR Cloning: On the General Use of Type IIS Restriction Enzymes for Cloning

The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert. A Type IIS endonuclease - a Body Double of the Type IIP enzyme - is used to generate the same protruding palindrom...

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Bibliographic Details
Main Authors: Tóth, Eszter, Huszár, Krisztina, Bencsura, Petra, Kulcsár, Péter István, Vodicska, Barbara, Nyeste, Antal, Welker, Zsombor, Tóth, Szilvia, Welker, Ervin
Format: Online
Language:English
Published: Public Library of Science 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949710/

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949710/

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