DNA methylome in spleen of avian pathogenic escherichia coli-challenged broilers and integration with mRNA expression
Avian pathogenic Escherichia coli (APEC) are responsible for heavy economic losses in poultry industry. Here we investigate DNA methylome of spleen and identify functional DNA methylation changes related to host response to APEC among groups of non-challenged chickens (NC), challenged with mild (MD)...
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| Format: | Online |
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Nature Publishing Group
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944351/ |
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pubmed-39443512014-03-06 DNA methylome in spleen of avian pathogenic escherichia coli-challenged broilers and integration with mRNA expression Xu, Haiping Zhu, Xuenong Hu, Yongsheng Li, Zhenhui Zhang, Xiquan Nie, Qinghua Article Avian pathogenic Escherichia coli (APEC) are responsible for heavy economic losses in poultry industry. Here we investigate DNA methylome of spleen and identify functional DNA methylation changes related to host response to APEC among groups of non-challenged chickens (NC), challenged with mild (MD) and severe pathology (SV). DNA methylation was enriched in the gene bodies and repeats. Promoter and CGIs are hypomethylated. Integration analysis revealed 22, 87, and 9 genes exhibiting inversely changed DNA methylation and gene expression in NC vs. MD, NC vs. SV, and MD vs. SV, respectively. IL8, IL2RB, and IL1RAPL1 were included. Gene network analysis suggested that besides inflammatory response, other networks and pathways such as organismal injury and abnormalities, cell signaling and molecular transport, are probably related to host response to APEC infection. Moreover, methylation changes in cell cycle processes might contribute to the lesion phenotype differences between MD and SV. Nature Publishing Group 2014-03-06 /pmc/articles/PMC3944351/ /pubmed/24599154 http://dx.doi.org/10.1038/srep04299 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Xu, Haiping Zhu, Xuenong Hu, Yongsheng Li, Zhenhui Zhang, Xiquan Nie, Qinghua |
| spellingShingle |
Xu, Haiping Zhu, Xuenong Hu, Yongsheng Li, Zhenhui Zhang, Xiquan Nie, Qinghua DNA methylome in spleen of avian pathogenic escherichia coli-challenged broilers and integration with mRNA expression |
| author_facet |
Xu, Haiping Zhu, Xuenong Hu, Yongsheng Li, Zhenhui Zhang, Xiquan Nie, Qinghua |
| author_sort |
Xu, Haiping |
| title |
DNA methylome in spleen of avian pathogenic escherichia coli-challenged broilers and integration with mRNA expression |
| title_short |
DNA methylome in spleen of avian pathogenic escherichia coli-challenged broilers and integration with mRNA expression |
| title_full |
DNA methylome in spleen of avian pathogenic escherichia coli-challenged broilers and integration with mRNA expression |
| title_fullStr |
DNA methylome in spleen of avian pathogenic escherichia coli-challenged broilers and integration with mRNA expression |
| title_full_unstemmed |
DNA methylome in spleen of avian pathogenic escherichia coli-challenged broilers and integration with mRNA expression |
| title_sort |
dna methylome in spleen of avian pathogenic escherichia coli-challenged broilers and integration with mrna expression |
| description |
Avian pathogenic Escherichia coli (APEC) are responsible for heavy economic losses in poultry industry. Here we investigate DNA methylome of spleen and identify functional DNA methylation changes related to host response to APEC among groups of non-challenged chickens (NC), challenged with mild (MD) and severe pathology (SV). DNA methylation was enriched in the gene bodies and repeats. Promoter and CGIs are hypomethylated. Integration analysis revealed 22, 87, and 9 genes exhibiting inversely changed DNA methylation and gene expression in NC vs. MD, NC vs. SV, and MD vs. SV, respectively. IL8, IL2RB, and IL1RAPL1 were included. Gene network analysis suggested that besides inflammatory response, other networks and pathways such as organismal injury and abnormalities, cell signaling and molecular transport, are probably related to host response to APEC infection. Moreover, methylation changes in cell cycle processes might contribute to the lesion phenotype differences between MD and SV. |
| publisher |
Nature Publishing Group |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944351/ |
| _version_ |
1612064979364610048 |