Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations

Anti-nuclear antibody (ANA) assay is a screening test used for almost all autoimmune rheumatic diseases, and in a number of these cases, it is a diagnostic/classification parameter. In addition, ANA is also a useful test for additional autoimmune disorders. The indirect immunofluorescence technique...

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Main Authors:	Meroni, Pier Luigi, Bizzaro, Nicola, Cavazzana, Ilaria, Borghi, Maria Orietta, Tincani, Angela
Format:	Online
Language:	English
Published:	BioMed Central 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3939809/

id	pubmed-3939809
recordtype	oai_dc
spelling	pubmed-39398092014-03-04 Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations Meroni, Pier Luigi Bizzaro, Nicola Cavazzana, Ilaria Borghi, Maria Orietta Tincani, Angela Commentary Anti-nuclear antibody (ANA) assay is a screening test used for almost all autoimmune rheumatic diseases, and in a number of these cases, it is a diagnostic/classification parameter. In addition, ANA is also a useful test for additional autoimmune disorders. The indirect immunofluorescence technique on monolayers of cultured epithelial cells is the current recommended method because it has higher sensitivity than solid phase assays. However, the technique is time-consuming and requires skilled operators. Automated ANA reading systems have recently been developed, which offer the advantage of faster and much easier performance as well as better harmonization in the interpretation of the results. Preliminary validation studies of these systems have given promising results in terms of analytical specificity and reproducibility. However, these techniques require further validation in clinical studies and need improvement in their recognition of mixed or less common staining patterns. BioMed Central 2014-03-03 /pmc/articles/PMC3939809/ /pubmed/24589329 http://dx.doi.org/10.1186/1741-7015-12-38 Text en Copyright © 2014 Meroni et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Meroni, Pier Luigi Bizzaro, Nicola Cavazzana, Ilaria Borghi, Maria Orietta Tincani, Angela
spellingShingle	Meroni, Pier Luigi Bizzaro, Nicola Cavazzana, Ilaria Borghi, Maria Orietta Tincani, Angela Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations
author_facet	Meroni, Pier Luigi Bizzaro, Nicola Cavazzana, Ilaria Borghi, Maria Orietta Tincani, Angela
author_sort	Meroni, Pier Luigi
title	Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations
title_short	Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations
title_full	Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations
title_fullStr	Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations
title_full_unstemmed	Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations
title_sort	automated tests of ana immunofluorescence as throughput autoantibody detection technology: strengths and limitations
description	Anti-nuclear antibody (ANA) assay is a screening test used for almost all autoimmune rheumatic diseases, and in a number of these cases, it is a diagnostic/classification parameter. In addition, ANA is also a useful test for additional autoimmune disorders. The indirect immunofluorescence technique on monolayers of cultured epithelial cells is the current recommended method because it has higher sensitivity than solid phase assays. However, the technique is time-consuming and requires skilled operators. Automated ANA reading systems have recently been developed, which offer the advantage of faster and much easier performance as well as better harmonization in the interpretation of the results. Preliminary validation studies of these systems have given promising results in terms of analytical specificity and reproducibility. However, these techniques require further validation in clinical studies and need improvement in their recognition of mixed or less common staining patterns.
publisher	BioMed Central
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3939809/
_version_	1612063473336844288

Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations

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