Production of Functional Gametes from Cryopreserved Primordial Germ Cells of the Japanese Quail
The Japanese quail (Coturnix japonica) is a valuable bird as both an experimental animal, for a wide range of scientific disciplines, and an agricultural animal, for the production of eggs and meat. Cryopreservation of PGCs would be a feasible strategy for the conservation of both male and female f...
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| Format: | Online |
| Language: | English |
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The Society for Reproduction and Development
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934152/ |
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pubmed-39341522014-03-06 Production of Functional Gametes from Cryopreserved Primordial Germ Cells of the Japanese Quail NAKAMURA, Yoshiaki TASAI, Mariko TAKEDA, Kumiko NIRASAWA, Keijiro TAGAMI, Takahiro Original Article The Japanese quail (Coturnix japonica) is a valuable bird as both an experimental animal, for a wide range of scientific disciplines, and an agricultural animal, for the production of eggs and meat. Cryopreservation of PGCs would be a feasible strategy for the conservation of both male and female fertility cells in Japanese quail. However, the effects of freeze-thaw treatment on viability, migration ability and germline transmission ability of quail PGCs still remain unclear. In the present study, male and female PGCs were isolated from the blood of 2-day-old embryos, which were cooled by slow freezing and then cryopreserved at –196 C for 77–185 days, respectively. The average recovery rate of PGCs after freeze-thawing was 47.0%. The viability of PGCs in the frozen group was significantly lower than that of the control group (P<0.05) (85.5% vs. 95.1%). Both fresh and Frozen-thawed PGCs that were intravascularly transplanted into recipient embryos migrated toward and were incorporated into recipient gonads, although the number of PGCs settled in the gonads was 48.5% lower in the frozen group than in the unfrozen control group (P<0.05). Genetic cross analysis revealed that one female and two male recipients produced live progeny derived from the frozen-thawed PGCs. The frequency of donor-derived offspring was slightly lower than that of unfrozen controls, but the difference was not significant (4.0 vs. 14.0%). These results revealed that freeze-thaw treatment causes a decrease in viability, migration ability and germline transmission ability of PGCs in quail. The Society for Reproduction and Development 2013-09-28 2013-12 /pmc/articles/PMC3934152/ /pubmed/24077020 http://dx.doi.org/10.1262/jrd.2013-065 Text en ©2013 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
NAKAMURA, Yoshiaki TASAI, Mariko TAKEDA, Kumiko NIRASAWA, Keijiro TAGAMI, Takahiro |
| spellingShingle |
NAKAMURA, Yoshiaki TASAI, Mariko TAKEDA, Kumiko NIRASAWA, Keijiro TAGAMI, Takahiro Production of Functional Gametes from Cryopreserved Primordial Germ Cells of the Japanese Quail |
| author_facet |
NAKAMURA, Yoshiaki TASAI, Mariko TAKEDA, Kumiko NIRASAWA, Keijiro TAGAMI, Takahiro |
| author_sort |
NAKAMURA, Yoshiaki |
| title |
Production of Functional Gametes from Cryopreserved Primordial Germ Cells
of the Japanese Quail |
| title_short |
Production of Functional Gametes from Cryopreserved Primordial Germ Cells
of the Japanese Quail |
| title_full |
Production of Functional Gametes from Cryopreserved Primordial Germ Cells
of the Japanese Quail |
| title_fullStr |
Production of Functional Gametes from Cryopreserved Primordial Germ Cells
of the Japanese Quail |
| title_full_unstemmed |
Production of Functional Gametes from Cryopreserved Primordial Germ Cells
of the Japanese Quail |
| title_sort |
production of functional gametes from cryopreserved primordial germ cells
of the japanese quail |
| description |
The Japanese quail (Coturnix japonica) is a valuable bird as both
an experimental animal, for a wide range of scientific disciplines, and an
agricultural animal, for the production of eggs and meat. Cryopreservation of PGCs
would be a feasible strategy for the conservation of both male and female fertility
cells in Japanese quail. However, the effects of freeze-thaw treatment on viability,
migration ability and germline transmission ability of quail PGCs still remain
unclear. In the present study, male and female PGCs were isolated from the blood of
2-day-old embryos, which were cooled by slow freezing and then cryopreserved at –196
C for 77–185 days, respectively. The average recovery rate of PGCs after
freeze-thawing was 47.0%. The viability of PGCs in the frozen group was significantly
lower than that of the control group (P<0.05) (85.5% vs. 95.1%).
Both fresh and Frozen-thawed PGCs that were intravascularly transplanted into
recipient embryos migrated toward and were incorporated into recipient gonads,
although the number of PGCs settled in the gonads was 48.5% lower in the frozen group
than in the unfrozen control group (P<0.05). Genetic cross analysis revealed that
one female and two male recipients produced live progeny derived from the
frozen-thawed PGCs. The frequency of donor-derived offspring was slightly lower than
that of unfrozen controls, but the difference was not significant (4.0
vs. 14.0%). These results revealed that freeze-thaw treatment
causes a decrease in viability, migration ability and germline transmission ability
of PGCs in quail. |
| publisher |
The Society for Reproduction and Development |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3934152/ |
| _version_ |
1612061716882915328 |