A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species

A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species

Objective(s): Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish betwee...

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Main Authors: Shafiei, Reza, Sarkari, Bahador, Moshfe, Abdolali
Format: Online
Language:English
Published: Mashhad University of Medical Sciences 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933804/
id pubmed-3933804
recordtype oai_dc
spelling pubmed-39338042014-02-25 A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species Shafiei, Reza Sarkari, Bahador Moshfe, Abdolali Original Article Objective(s): Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica, based on profiles of RFLP, produced by effect of endonucleases on ITS2 of the ribosomal DNA genes from these two species. Mashhad University of Medical Sciences 2013-12 /pmc/articles/PMC3933804/ /pubmed/24570833 Text en © 2013: Iranian Journal of Basic Medical Sciences This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Shafiei, Reza
Sarkari, Bahador
Moshfe, Abdolali
spellingShingle Shafiei, Reza
Sarkari, Bahador
Moshfe, Abdolali
A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species
author_facet Shafiei, Reza
Sarkari, Bahador
Moshfe, Abdolali
author_sort Shafiei, Reza
title A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species
title_short A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species
title_full A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species
title_fullStr A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species
title_full_unstemmed A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species
title_sort consistent pcr-rflp assay based on its-2 ribosomal dna for differentiation of fasciola species
description Objective(s): Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica, based on profiles of RFLP, produced by effect of endonucleases on ITS2 of the ribosomal DNA genes from these two species.
publisher Mashhad University of Medical Sciences
publishDate 2013
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933804/
_version_ 1612061601096007680

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