Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion

The aim of the present study was to provide a simple method of establishing a rat model for focal cerebral ischemia-reperfusion (FCIR). The suture-occluded method was used to establish FCIR in male Sprague-Dawley rats. An incision was made over the bifurcation of the common carotid artery (CCA), thr...

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Main Authors:	ZHANG, PING, HUANG, ZHEN, YAN, HAI-QING, SU, LIN-LIN, GUI, YONG-KUN, LV, HAI-XIA, ZHU, BIN, LI, TONG
Format:	Online
Language:	English
Published:	D.A. Spandidos 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3919944/

id	pubmed-3919944
recordtype	oai_dc
spelling	pubmed-39199442014-02-11 Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion ZHANG, PING HUANG, ZHEN YAN, HAI-QING SU, LIN-LIN GUI, YONG-KUN LV, HAI-XIA ZHU, BIN LI, TONG Articles The aim of the present study was to provide a simple method of establishing a rat model for focal cerebral ischemia-reperfusion (FCIR). The suture-occluded method was used to establish FCIR in male Sprague-Dawley rats. An incision was made over the bifurcation of the common carotid artery (CCA), through which a suture was inserted up to the internal carotid artery (ICA). The suture remained in the skin subsequent to model establishment and was withdrawn to the CCA to enable reperfusion. The reliability of the rat model was assessed via analysis of nerve function, tetrazolium (TTC) staining and pathological examination. Following FCIR in rats, the resulting neurological impairments were observed. TTC staining revealed infarcts and pathological examination revealed typical pathological changes. This modified method was simple, reliable and, therefore, may be used to investigate FCIR. D.A. Spandidos 2014-03 2014-01-10 /pmc/articles/PMC3919944/ /pubmed/24520263 http://dx.doi.org/10.3892/etm.2014.1483 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	ZHANG, PING HUANG, ZHEN YAN, HAI-QING SU, LIN-LIN GUI, YONG-KUN LV, HAI-XIA ZHU, BIN LI, TONG
spellingShingle	ZHANG, PING HUANG, ZHEN YAN, HAI-QING SU, LIN-LIN GUI, YONG-KUN LV, HAI-XIA ZHU, BIN LI, TONG Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion
author_facet	ZHANG, PING HUANG, ZHEN YAN, HAI-QING SU, LIN-LIN GUI, YONG-KUN LV, HAI-XIA ZHU, BIN LI, TONG
author_sort	ZHANG, PING
title	Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion
title_short	Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion
title_full	Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion
title_fullStr	Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion
title_full_unstemmed	Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion
title_sort	improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion
description	The aim of the present study was to provide a simple method of establishing a rat model for focal cerebral ischemia-reperfusion (FCIR). The suture-occluded method was used to establish FCIR in male Sprague-Dawley rats. An incision was made over the bifurcation of the common carotid artery (CCA), through which a suture was inserted up to the internal carotid artery (ICA). The suture remained in the skin subsequent to model establishment and was withdrawn to the CCA to enable reperfusion. The reliability of the rat model was assessed via analysis of nerve function, tetrazolium (TTC) staining and pathological examination. Following FCIR in rats, the resulting neurological impairments were observed. TTC staining revealed infarcts and pathological examination revealed typical pathological changes. This modified method was simple, reliable and, therefore, may be used to investigate FCIR.
publisher	D.A. Spandidos
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3919944/
_version_	1612056937150545920

Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion

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