Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection

Polystyrene (PS) nanoparticle (NP) copolymerized with acrylic acid (AA) and coloured monomer, i.e. 2,3,6,7-tetra(2,2′-bithiophene)-1,4,5,8-naphthalenetetracarboxylic-N,N′-di(2-methylallyl)-bisimide (ALN8T), was synthesized via the miniemulsion polymerization. Before applying for malaria antigen dete...

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Main Authors:	Thiramanas, Raweewan, Jangpatarapongsa, Kulachart, Asawapirom, Udom, Tangboriboonrat, Pramuan, Polpanich, Duangporn
Format:	Online
Language:	English
Published:	John Wiley & Sons Ltd 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917475/

id	pubmed-3917475
recordtype	oai_dc
spelling	pubmed-39174752014-02-12 Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection Thiramanas, Raweewan Jangpatarapongsa, Kulachart Asawapirom, Udom Tangboriboonrat, Pramuan Polpanich, Duangporn Research Articles Polystyrene (PS) nanoparticle (NP) copolymerized with acrylic acid (AA) and coloured monomer, i.e. 2,3,6,7-tetra(2,2′-bithiophene)-1,4,5,8-naphthalenetetracarboxylic-N,N′-di(2-methylallyl)-bisimide (ALN8T), was synthesized via the miniemulsion polymerization. Before applying for malaria antigen detection, the blue NP was conjugated with human polyclonal malaria IgG antibody (Ab) specific to Plasmodium falciparum. For the conjugation, three methods, i.e. physical adsorption, covalent coupling and affinity binding via streptavidin (SA) and biotin interaction, were employed. The optimum ratio of Ab to NPs used in each immobilization procedure and the latex agglutination test based on the reaction between Ab conjugated NPs and malaria patient plasma were investigated. All Ab–latex conjugates provided the high sensitivity for the detection of P. falciparum malaria plasma. The highest specificity to P. falciparum was obtained from using Ab–NPs conjugated via the SA–biotin interaction. John Wiley & Sons Ltd 2013-07 2013-06-11 /pmc/articles/PMC3917475/ /pubmed/23298152 http://dx.doi.org/10.1111/1751-7915.12021 Text en © 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Thiramanas, Raweewan Jangpatarapongsa, Kulachart Asawapirom, Udom Tangboriboonrat, Pramuan Polpanich, Duangporn
spellingShingle	Thiramanas, Raweewan Jangpatarapongsa, Kulachart Asawapirom, Udom Tangboriboonrat, Pramuan Polpanich, Duangporn Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection
author_facet	Thiramanas, Raweewan Jangpatarapongsa, Kulachart Asawapirom, Udom Tangboriboonrat, Pramuan Polpanich, Duangporn
author_sort	Thiramanas, Raweewan
title	Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection
title_short	Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection
title_full	Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection
title_fullStr	Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection
title_full_unstemmed	Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection
title_sort	sensitivity and specificity of ps/aa-modified nanoparticles used in malaria detection
description	Polystyrene (PS) nanoparticle (NP) copolymerized with acrylic acid (AA) and coloured monomer, i.e. 2,3,6,7-tetra(2,2′-bithiophene)-1,4,5,8-naphthalenetetracarboxylic-N,N′-di(2-methylallyl)-bisimide (ALN8T), was synthesized via the miniemulsion polymerization. Before applying for malaria antigen detection, the blue NP was conjugated with human polyclonal malaria IgG antibody (Ab) specific to Plasmodium falciparum. For the conjugation, three methods, i.e. physical adsorption, covalent coupling and affinity binding via streptavidin (SA) and biotin interaction, were employed. The optimum ratio of Ab to NPs used in each immobilization procedure and the latex agglutination test based on the reaction between Ab conjugated NPs and malaria patient plasma were investigated. All Ab–latex conjugates provided the high sensitivity for the detection of P. falciparum malaria plasma. The highest specificity to P. falciparum was obtained from using Ab–NPs conjugated via the SA–biotin interaction.
publisher	John Wiley & Sons Ltd
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917475/
_version_	1612056236780421120

Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection

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