Identification of germline transcriptional regulatory elements in Aedes aegypti

The mosquito Aedes aegypti is the principal vector for the yellow fever and dengue viruses, and is also responsible for recent outbreaks of the alphavirus chikungunya. Vector control strategies utilizing engineered gene drive systems are being developed as a means of replacing wild, pathogen transmi...

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Main Authors:	Akbari, Omar S., Papathanos, Philippos A., Sandler, Jeremy E., Kennedy, Katie, Hay, Bruce A.
Format:	Online
Language:	English
Published:	Nature Publishing Group 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912481/

id	pubmed-3912481
recordtype	oai_dc
spelling	pubmed-39124812014-02-04 Identification of germline transcriptional regulatory elements in Aedes aegypti Akbari, Omar S. Papathanos, Philippos A. Sandler, Jeremy E. Kennedy, Katie Hay, Bruce A. Article The mosquito Aedes aegypti is the principal vector for the yellow fever and dengue viruses, and is also responsible for recent outbreaks of the alphavirus chikungunya. Vector control strategies utilizing engineered gene drive systems are being developed as a means of replacing wild, pathogen transmitting mosquitoes with individuals refractory to disease transmission, or bringing about population suppression. Several of these systems, including Medea, UDMEL, and site-specific nucleases, which can be used to drive genes into populations or bring about population suppression, utilize transcriptional regulatory elements that drive germline-specific expression. Here we report the identification of multiple regulatory elements able to drive gene expression specifically in the female germline, or in the male and female germline, in the mosquito Aedes aegypti. These elements can also be used as tools with which to probe the roles of specific genes in germline function and in the early embryo, through overexpression or RNA interference. Nature Publishing Group 2014-02-04 /pmc/articles/PMC3912481/ /pubmed/24492376 http://dx.doi.org/10.1038/srep03954 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Akbari, Omar S. Papathanos, Philippos A. Sandler, Jeremy E. Kennedy, Katie Hay, Bruce A.
spellingShingle	Akbari, Omar S. Papathanos, Philippos A. Sandler, Jeremy E. Kennedy, Katie Hay, Bruce A. Identification of germline transcriptional regulatory elements in Aedes aegypti
author_facet	Akbari, Omar S. Papathanos, Philippos A. Sandler, Jeremy E. Kennedy, Katie Hay, Bruce A.
author_sort	Akbari, Omar S.
title	Identification of germline transcriptional regulatory elements in Aedes aegypti
title_short	Identification of germline transcriptional regulatory elements in Aedes aegypti
title_full	Identification of germline transcriptional regulatory elements in Aedes aegypti
title_fullStr	Identification of germline transcriptional regulatory elements in Aedes aegypti
title_full_unstemmed	Identification of germline transcriptional regulatory elements in Aedes aegypti
title_sort	identification of germline transcriptional regulatory elements in aedes aegypti
description	The mosquito Aedes aegypti is the principal vector for the yellow fever and dengue viruses, and is also responsible for recent outbreaks of the alphavirus chikungunya. Vector control strategies utilizing engineered gene drive systems are being developed as a means of replacing wild, pathogen transmitting mosquitoes with individuals refractory to disease transmission, or bringing about population suppression. Several of these systems, including Medea, UDMEL, and site-specific nucleases, which can be used to drive genes into populations or bring about population suppression, utilize transcriptional regulatory elements that drive germline-specific expression. Here we report the identification of multiple regulatory elements able to drive gene expression specifically in the female germline, or in the male and female germline, in the mosquito Aedes aegypti. These elements can also be used as tools with which to probe the roles of specific genes in germline function and in the early embryo, through overexpression or RNA interference.
publisher	Nature Publishing Group
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912481/
_version_	1612054287915941888

Identification of germline transcriptional regulatory elements in Aedes aegypti

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