Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides

The binding of monosaccharides and short peptides to lymphocyte receptors (human CD69 and rat NKR-P1A) was first reported in 1994 and then in a number of subsequent publications. Based on this observation, numerous potentially high-affinity saccharide ligands have been synthesized over the last two...

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Main Authors:	Rozbeský, Daniel, Krejzová, Jana, Křenek, Karel, Prchal, Jan, Hrabal, Richard, Kožíšek, Milan, Weignerová, Lenka, Fiore, Michele, Dumy, Pascal, Renaudet, Olivier, Křen, Vladimír
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907868/

id	pubmed-3907868
recordtype	oai_dc
spelling	pubmed-39078682014-01-31 Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides Rozbeský, Daniel Krejzová, Jana Křenek, Karel Prchal, Jan Hrabal, Richard Kožíšek, Milan Weignerová, Lenka Fiore, Michele Dumy, Pascal Renaudet, Olivier Křen, Vladimír Article The binding of monosaccharides and short peptides to lymphocyte receptors (human CD69 and rat NKR-P1A) was first reported in 1994 and then in a number of subsequent publications. Based on this observation, numerous potentially high-affinity saccharide ligands have been synthesized over the last two decades in order to utilize their potential in antitumor therapy. Due to significant inconsistencies in their reported binding properties, we decided to re-examine the interaction between multiple ligands and CD69 or NKR-P1A. Using NMR titration and isothermal titration calorimetry we were unable to detect the binding of the tested ligands such as N-acetyl-d-hexosamines and oligopeptides to both receptors, which contradicts the previous observations published in more than twenty papers over the last fifteen years. Molecular Diversity Preservation International (MDPI) 2014-01-17 /pmc/articles/PMC3907868/ /pubmed/24445261 http://dx.doi.org/10.3390/ijms15011271 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Rozbeský, Daniel Krejzová, Jana Křenek, Karel Prchal, Jan Hrabal, Richard Kožíšek, Milan Weignerová, Lenka Fiore, Michele Dumy, Pascal Renaudet, Olivier Křen, Vladimír
spellingShingle	Rozbeský, Daniel Krejzová, Jana Křenek, Karel Prchal, Jan Hrabal, Richard Kožíšek, Milan Weignerová, Lenka Fiore, Michele Dumy, Pascal Renaudet, Olivier Křen, Vladimír Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides
author_facet	Rozbeský, Daniel Krejzová, Jana Křenek, Karel Prchal, Jan Hrabal, Richard Kožíšek, Milan Weignerová, Lenka Fiore, Michele Dumy, Pascal Renaudet, Olivier Křen, Vladimír
author_sort	Rozbeský, Daniel
title	Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides
title_short	Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides
title_full	Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides
title_fullStr	Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides
title_full_unstemmed	Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides
title_sort	re-evaluation of binding properties of recombinant lymphocyte receptors nkr-p1a and cd69 to chemically synthesized glycans and peptides
description	The binding of monosaccharides and short peptides to lymphocyte receptors (human CD69 and rat NKR-P1A) was first reported in 1994 and then in a number of subsequent publications. Based on this observation, numerous potentially high-affinity saccharide ligands have been synthesized over the last two decades in order to utilize their potential in antitumor therapy. Due to significant inconsistencies in their reported binding properties, we decided to re-examine the interaction between multiple ligands and CD69 or NKR-P1A. Using NMR titration and isothermal titration calorimetry we were unable to detect the binding of the tested ligands such as N-acetyl-d-hexosamines and oligopeptides to both receptors, which contradicts the previous observations published in more than twenty papers over the last fifteen years.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907868/
_version_	1612053187605299200

Re-Evaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides

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