Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability
We observed significant changes in the elemental and intact polar lipid (IPL) composition of the archaeon Thermococcus kodakarensis (KOD1) in response to growth stage and phosphorus supply. Reducing the amount of organic supplements and phosphate in growth media resulted in significant decreases in...
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Frontiers Media S.A.
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906577/ |
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pubmed-39065772014-02-12 Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability Meador, Travis B. Gagen, Emma J. Loscar, Michael E. Goldhammer, Tobias Yoshinaga, Marcos Y. Wendt, Jenny Thomm, Michael Hinrichs, Kai-Uwe Microbiology We observed significant changes in the elemental and intact polar lipid (IPL) composition of the archaeon Thermococcus kodakarensis (KOD1) in response to growth stage and phosphorus supply. Reducing the amount of organic supplements and phosphate in growth media resulted in significant decreases in cell size and cellular quotas of carbon (C), nitrogen (N), and phosphorus (P), which coincided with significant increases in cellular IPL quota and IPLs comprising multiple P atoms and hexose moieties. Relatively more cellular P was stored as IPLs in P-limited cells (2–8%) compared to control cells (<0.8%). We also identified a specific IPL biomarker containing a phosphatidyl-N-acetylhexoseamine headgroup that was relatively enriched during rapid cell division. These observations serve as empirical evidence of IPL adaptations in Archaea that will help to interpret the distribution of these biomarkers in natural systems. The reported cell quotas of C, N, and P represent the first such data for a specific archaeon and suggest that thermophiles are C-rich compared to the cell carbon-to-volume relationship reported for planktonic bacteria. Frontiers Media S.A. 2014-01-30 /pmc/articles/PMC3906577/ /pubmed/24523718 http://dx.doi.org/10.3389/fmicb.2014.00010 Text en Copyright © 2014 Meador, Gagen, Loscar, Goldhammer, Yoshinaga, Wendt, Thomm and Hinrichs. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Meador, Travis B. Gagen, Emma J. Loscar, Michael E. Goldhammer, Tobias Yoshinaga, Marcos Y. Wendt, Jenny Thomm, Michael Hinrichs, Kai-Uwe |
| spellingShingle |
Meador, Travis B. Gagen, Emma J. Loscar, Michael E. Goldhammer, Tobias Yoshinaga, Marcos Y. Wendt, Jenny Thomm, Michael Hinrichs, Kai-Uwe Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability |
| author_facet |
Meador, Travis B. Gagen, Emma J. Loscar, Michael E. Goldhammer, Tobias Yoshinaga, Marcos Y. Wendt, Jenny Thomm, Michael Hinrichs, Kai-Uwe |
| author_sort |
Meador, Travis B. |
| title |
Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability |
| title_short |
Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability |
| title_full |
Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability |
| title_fullStr |
Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability |
| title_full_unstemmed |
Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability |
| title_sort |
thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability |
| description |
We observed significant changes in the elemental and intact polar lipid (IPL) composition of the archaeon Thermococcus kodakarensis (KOD1) in response to growth stage and phosphorus supply. Reducing the amount of organic supplements and phosphate in growth media resulted in significant decreases in cell size and cellular quotas of carbon (C), nitrogen (N), and phosphorus (P), which coincided with significant increases in cellular IPL quota and IPLs comprising multiple P atoms and hexose moieties. Relatively more cellular P was stored as IPLs in P-limited cells (2–8%) compared to control cells (<0.8%). We also identified a specific IPL biomarker containing a phosphatidyl-N-acetylhexoseamine headgroup that was relatively enriched during rapid cell division. These observations serve as empirical evidence of IPL adaptations in Archaea that will help to interpret the distribution of these biomarkers in natural systems. The reported cell quotas of C, N, and P represent the first such data for a specific archaeon and suggest that thermophiles are C-rich compared to the cell carbon-to-volume relationship reported for planktonic bacteria. |
| publisher |
Frontiers Media S.A. |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906577/ |
| _version_ |
1612052744819965952 |