Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate

Abstract The centre–surround organisation of receptive fields is a feature of most retinal ganglion cells (RGCs) and is critical for spatial discrimination and contrast detection. Although lateral inhibitory processes are known to be important in generating the receptive field surround, the contribu...

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Main Authors:	Protti, D A, Di Marco, S, Huang, J Y, Vonhoff, C R, Nguyen, V, Solomon, S G
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903351/

id	pubmed-3903351
recordtype	oai_dc
spelling	pubmed-39033512014-11-18 Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate Protti, D A Di Marco, S Huang, J Y Vonhoff, C R Nguyen, V Solomon, S G Neuroscience: Cellular/Molecular Abstract The centre–surround organisation of receptive fields is a feature of most retinal ganglion cells (RGCs) and is critical for spatial discrimination and contrast detection. Although lateral inhibitory processes are known to be important in generating the receptive field surround, the contribution of each of the two synaptic layers in the primate retina remains unclear. Here we studied the spatial organisation of excitatory and inhibitory synaptic inputs onto ON and OFF ganglion cells in the primate retina. All RGCs showed an increase in excitation in response to stimulus of preferred polarity. Inhibition onto RGCs comprised two types of responses to preferred polarity: some RGCs showed an increase in inhibition whilst others showed removal of tonic inhibition. Excitatory inputs were strongly spatially tuned but inhibitory inputs showed more variable organisation: in some neurons they were as strongly tuned as excitation, and in others inhibitory inputs showed no spatial tuning. We targeted one source of inner retinal inhibition by functionally ablating spiking amacrine cells with bath application of tetrodotoxin (TTX). TTX significantly reduced the spatial tuning of excitatory inputs. In addition, TTX reduced inhibition onto those RGCs where a stimulus of preferred polarity increased inhibition. Reconstruction of the spatial tuning properties by somatic injection of excitatory and inhibitory synaptic conductances verified that TTX-mediated inhibition onto bipolar cells increases the strength of the surround in RGC spiking output. These results indicate that in the primate retina inhibitory mechanisms in the inner plexiform layer sharpen the spatial tuning of ganglion cells. Blackwell Publishing Ltd 2014-01-01 2013-09-16 /pmc/articles/PMC3903351/ /pubmed/24042496 http://dx.doi.org/10.1113/jphysiol.2013.257352 Text en © 2013 The Authors. The Journal of Physiology © 2013 The Physiological Society
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Protti, D A Di Marco, S Huang, J Y Vonhoff, C R Nguyen, V Solomon, S G
spellingShingle	Protti, D A Di Marco, S Huang, J Y Vonhoff, C R Nguyen, V Solomon, S G Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate
author_facet	Protti, D A Di Marco, S Huang, J Y Vonhoff, C R Nguyen, V Solomon, S G
author_sort	Protti, D A
title	Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate
title_short	Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate
title_full	Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate
title_fullStr	Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate
title_full_unstemmed	Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate
title_sort	inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate
description	Abstract The centre–surround organisation of receptive fields is a feature of most retinal ganglion cells (RGCs) and is critical for spatial discrimination and contrast detection. Although lateral inhibitory processes are known to be important in generating the receptive field surround, the contribution of each of the two synaptic layers in the primate retina remains unclear. Here we studied the spatial organisation of excitatory and inhibitory synaptic inputs onto ON and OFF ganglion cells in the primate retina. All RGCs showed an increase in excitation in response to stimulus of preferred polarity. Inhibition onto RGCs comprised two types of responses to preferred polarity: some RGCs showed an increase in inhibition whilst others showed removal of tonic inhibition. Excitatory inputs were strongly spatially tuned but inhibitory inputs showed more variable organisation: in some neurons they were as strongly tuned as excitation, and in others inhibitory inputs showed no spatial tuning. We targeted one source of inner retinal inhibition by functionally ablating spiking amacrine cells with bath application of tetrodotoxin (TTX). TTX significantly reduced the spatial tuning of excitatory inputs. In addition, TTX reduced inhibition onto those RGCs where a stimulus of preferred polarity increased inhibition. Reconstruction of the spatial tuning properties by somatic injection of excitatory and inhibitory synaptic conductances verified that TTX-mediated inhibition onto bipolar cells increases the strength of the surround in RGC spiking output. These results indicate that in the primate retina inhibitory mechanisms in the inner plexiform layer sharpen the spatial tuning of ganglion cells.
publisher	Blackwell Publishing Ltd
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903351/
_version_	1612051660631179264

Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate

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