Unblocking the Sink: Improved CID-Based Analysis of Phosphorylated Peptides by Enzymatic Removal of the Basic C-Terminal Residue
A one-step enzymatic reaction for improving the collision-induced dissociation (CID)-based tandem mass spectrometry (MS/MS) analysis of phosphorylated peptides in an ion trap is presented. Carboxypeptidase-B (CBP-B) was used to selectively remove C-terminal arginine or lysine residues from phosphory...
| Main Authors: | , , |
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| Format: | Online |
| Language: | English |
| Published: |
Springer US
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899453/ |