Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis

The small GTP-binding protein Rab8 is known to play an essential role in intracellular transport and cilia formation. We have previously demonstrated that Rab8a is required for localising apical markers in various organisms. Rab8a has a closely related isoform, Rab8b. To determine whether Rab8b can...

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Main Authors:	Sato, Takashi, Iwano, Tomohiko, Kunii, Masataka, Matsuda, Shinji, Mizuguchi, Rumiko, Jung, Yongwook, Hagiwara, Haruo, Yoshihara, Yoshihiro, Yuzaki, Michisuke, Harada, Reiko, Harada, Akihiro
Format:	Online
Language:	English
Published:	The Company of Biologists 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898603/

id	pubmed-3898603
recordtype	oai_dc
spelling	pubmed-38986032015-01-15 Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis Sato, Takashi Iwano, Tomohiko Kunii, Masataka Matsuda, Shinji Mizuguchi, Rumiko Jung, Yongwook Hagiwara, Haruo Yoshihara, Yoshihiro Yuzaki, Michisuke Harada, Reiko Harada, Akihiro Research Article The small GTP-binding protein Rab8 is known to play an essential role in intracellular transport and cilia formation. We have previously demonstrated that Rab8a is required for localising apical markers in various organisms. Rab8a has a closely related isoform, Rab8b. To determine whether Rab8b can compensate for Rab8a, we generated Rab8b-knockout mice. Although the Rab8b-knockout mice did not display an overt phenotype, Rab8a and Rab8b double-knockout mice exhibited mislocalisation of apical markers and died earlier than Rab8a-knockout mice. The apical markers accumulated in three intracellular patterns in the double-knockout mice. However, the localisation of basolateral and/or dendritic markers of the double-knockout mice seemed normal. The morphology and the length of various primary and/or motile cilia, and the frequency of ciliated cells appeared to be identical in control and double-knockout mice. However, an additional knockdown of Rab10 in double-knockout cells greatly reduced the percentage of ciliated cells. Our results highlight the compensatory effect of Rab8a and Rab8b in apical transport, and the complexity of the apical transport process. In addition, neither Rab8a nor Rab8b are required for basolateral and/or dendritic transport. However, simultaneous loss of Rab8a and Rab8b has little effect on ciliogenesis, whereas additional loss of Rab10 greatly affects ciliogenesis. The Company of Biologists 2014-01-15 /pmc/articles/PMC3898603/ /pubmed/24213529 http://dx.doi.org/10.1242/jcs.136903 Text en © 2014. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Sato, Takashi Iwano, Tomohiko Kunii, Masataka Matsuda, Shinji Mizuguchi, Rumiko Jung, Yongwook Hagiwara, Haruo Yoshihara, Yoshihiro Yuzaki, Michisuke Harada, Reiko Harada, Akihiro
spellingShingle	Sato, Takashi Iwano, Tomohiko Kunii, Masataka Matsuda, Shinji Mizuguchi, Rumiko Jung, Yongwook Hagiwara, Haruo Yoshihara, Yoshihiro Yuzaki, Michisuke Harada, Reiko Harada, Akihiro Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis
author_facet	Sato, Takashi Iwano, Tomohiko Kunii, Masataka Matsuda, Shinji Mizuguchi, Rumiko Jung, Yongwook Hagiwara, Haruo Yoshihara, Yoshihiro Yuzaki, Michisuke Harada, Reiko Harada, Akihiro
author_sort	Sato, Takashi
title	Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis
title_short	Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis
title_full	Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis
title_fullStr	Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis
title_full_unstemmed	Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis
title_sort	rab8a and rab8b are essential for several apical transport pathways but insufficient for ciliogenesis
description	The small GTP-binding protein Rab8 is known to play an essential role in intracellular transport and cilia formation. We have previously demonstrated that Rab8a is required for localising apical markers in various organisms. Rab8a has a closely related isoform, Rab8b. To determine whether Rab8b can compensate for Rab8a, we generated Rab8b-knockout mice. Although the Rab8b-knockout mice did not display an overt phenotype, Rab8a and Rab8b double-knockout mice exhibited mislocalisation of apical markers and died earlier than Rab8a-knockout mice. The apical markers accumulated in three intracellular patterns in the double-knockout mice. However, the localisation of basolateral and/or dendritic markers of the double-knockout mice seemed normal. The morphology and the length of various primary and/or motile cilia, and the frequency of ciliated cells appeared to be identical in control and double-knockout mice. However, an additional knockdown of Rab10 in double-knockout cells greatly reduced the percentage of ciliated cells. Our results highlight the compensatory effect of Rab8a and Rab8b in apical transport, and the complexity of the apical transport process. In addition, neither Rab8a nor Rab8b are required for basolateral and/or dendritic transport. However, simultaneous loss of Rab8a and Rab8b has little effect on ciliogenesis, whereas additional loss of Rab10 greatly affects ciliogenesis.
publisher	The Company of Biologists
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898603/
_version_	1612050039278927872

Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis

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