Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs

An experiment was conducted to determine how an E. coli challenge and dietary clays affect the intestinal barrier of pigs. Two groups of 32 pigs (initial BW: 6.9 ± 1.0 kg) were distributed in a 2 × 4 factorial arrangement of a randomized complete block design (2 challenge treatments: sham or E. coli...

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Main Authors:	Almeida, Juliana Abranches Soares, Liu, Yanhong, Song, Minho, Lee, Jeong Jae, Gaskins, H Rex, Maddox, Carol Wolfgang, Osuna, Orlando, Pettigrew, James Eugene
Format:	Online
Language:	English
Published:	BioMed Central 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897994/

id	pubmed-3897994
recordtype	oai_dc
spelling	pubmed-38979942014-01-23 Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs Almeida, Juliana Abranches Soares Liu, Yanhong Song, Minho Lee, Jeong Jae Gaskins, H Rex Maddox, Carol Wolfgang Osuna, Orlando Pettigrew, James Eugene Research An experiment was conducted to determine how an E. coli challenge and dietary clays affect the intestinal barrier of pigs. Two groups of 32 pigs (initial BW: 6.9 ± 1.0 kg) were distributed in a 2 × 4 factorial arrangement of a randomized complete block design (2 challenge treatments: sham or E. coli, and 4 dietary treatments: control, 0.3% smectite A, 0.3% smectite B and 0.3% zeolite), with 8 replicates total. Diarrhea score, growth performance, goblet cell size and number, bacterial translocation from intestinal lumen to lymph nodes, intestinal morphology, and relative amounts of sulfo and sialo mucins were measured. The E. coli challenge reduced performance, increased goblet cell size and number in the ileum, increased bacterial translocation from the intestinal lumen to the lymph nodes, and increased ileal crypt depth. One of the clays (smectite A) tended to increase goblet cell size in ileum, which may indicate enhanced protection. In conclusion, E. coli infection degrades intestinal barrier integrity but smectite A may enhance it. BioMed Central 2013-12-20 /pmc/articles/PMC3897994/ /pubmed/24359581 http://dx.doi.org/10.1186/2049-1891-4-52 Text en Copyright © 2013 Almeida et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Almeida, Juliana Abranches Soares Liu, Yanhong Song, Minho Lee, Jeong Jae Gaskins, H Rex Maddox, Carol Wolfgang Osuna, Orlando Pettigrew, James Eugene
spellingShingle	Almeida, Juliana Abranches Soares Liu, Yanhong Song, Minho Lee, Jeong Jae Gaskins, H Rex Maddox, Carol Wolfgang Osuna, Orlando Pettigrew, James Eugene Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs
author_facet	Almeida, Juliana Abranches Soares Liu, Yanhong Song, Minho Lee, Jeong Jae Gaskins, H Rex Maddox, Carol Wolfgang Osuna, Orlando Pettigrew, James Eugene
author_sort	Almeida, Juliana Abranches Soares
title	Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs
title_short	Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs
title_full	Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs
title_fullStr	Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs
title_full_unstemmed	Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs
title_sort	escherichia coli challenge and one type of smectite alter intestinal barrier of pigs
description	An experiment was conducted to determine how an E. coli challenge and dietary clays affect the intestinal barrier of pigs. Two groups of 32 pigs (initial BW: 6.9 ± 1.0 kg) were distributed in a 2 × 4 factorial arrangement of a randomized complete block design (2 challenge treatments: sham or E. coli, and 4 dietary treatments: control, 0.3% smectite A, 0.3% smectite B and 0.3% zeolite), with 8 replicates total. Diarrhea score, growth performance, goblet cell size and number, bacterial translocation from intestinal lumen to lymph nodes, intestinal morphology, and relative amounts of sulfo and sialo mucins were measured. The E. coli challenge reduced performance, increased goblet cell size and number in the ileum, increased bacterial translocation from the intestinal lumen to the lymph nodes, and increased ileal crypt depth. One of the clays (smectite A) tended to increase goblet cell size in ileum, which may indicate enhanced protection. In conclusion, E. coli infection degrades intestinal barrier integrity but smectite A may enhance it.
publisher	BioMed Central
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897994/
_version_	1612049757603102720

Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs

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